Skip to main content
. Author manuscript; available in PMC: 2017 Jan 15.
Published in final edited form as: Virology. 2015 Dec 4;488:271–277. doi: 10.1016/j.virol.2015.11.022

Figure 1. Effect of SAMHD1 T592 phosphorylation on cellular dATP levels.

Figure 1

U937 cells were transduced with lentiviral particles encoding either WT SAMHD1, the indicated mutants, or an empty vector. Following puromycin selection, cells were differentiated overnight with 10 ng/ml PMA. A) Total cell extracts from differentiated U937 stable cell lines were separated by SDS-PAGE and subjected to immunoblotting for SAMHD1 and actin as indicated. B) Differentiated cells were infected with increasing amounts of VSV-G pseudotyped HIV-1-GFP (as described in (Welbourn et al., 2013)) and the percent infection (% GFP- positive cells) was determined by flow cytometry 48 h later. C) Cellular dNTP levels were isolated at time of infection and amounts of dATP present per million cells were determined using a polymerase based assay described in the Methods section. Results in panels A and B are representative of at least 3 independent experiments. Error bars in panel C represent the mean and standard deviation of quantitation from at least 3 independently generated cell lines.