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. 2015 Jul 14;68(2):241–253. doi: 10.1111/his.12745

Figure 2.

Figure 2

Waterfall‐plot illustrating the distribution of MET amplification and hepatocyte growth factor receptor (MET) immunostaining among 55 patients. The waterfall plot shows case‐by‐case the results of chromogenic in‐situ hybridization (MET‐ CISH; logarithmic data presentation), and immunohistochemistry (MET‐IHC; liner data presentation) for patients belonging to group A (≥10% MET amplification), group B (<10% MET amplification), group C (MET‐IHC 3+ without MET amplification) and group D (MET‐IHC 2+ without MET amplification). Note that the different colours at the bottom illustrate the different staining intensities: MET‐IHC 3+ (red), MET‐IHC 2+ (green), MET‐IHC 1+ (blue) and MET‐IHC 0 (black). The sum total of all staining intensities found in a single case always added to a total HistoScore (Hscore) of 100% according to the following formula: P (MET‐IHC 0) + P (MET‐IHC 1+) + P (MET‐IHC 2+) + P (MET‐IHC 3+) = 100%. The yellow line marks a 10% cutoff, which is used, for example, to classify HER2/neu as either positive (above) or negative (below).