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. 1970 Mar;23(2):149–155. doi: 10.1136/jcp.23.2.149

Investigations and preparation of fibrinogencoated tanned sheep red cells

P C Das 1,2
PMCID: PMC474480  PMID: 5463737

Abstract

The preparation and investigations of tanned sheep red cells coated with human fibrinogen are presented in detail and noted variables are discussed. Both tanning and coating are quick processes. Higher concentrations of tannic acid improved the sensitivity of the cells but required an increased concentration of stabilizing serum to prevent autoagglutination. Fibrinogen concentration above 2 μg/ml had no significant effect, although a concentration above 100 μg/ml sometimes produced cells with incomplete patterns. The pH of the reaction media may be varied widely, although tanning and coating at a more alkaline pH yielded cells showing an indistinct pattern. Cells prepared by this method can be stored for at least six months.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allison A. C. Some effects of pharmacologically active compounds on membranes. Br Med Bull. 1968 May;24(2):135–140. doi: 10.1093/oxfordjournals.bmb.a070615. [DOI] [PubMed] [Google Scholar]
  2. BOYDEN S. V., SORKIN E. A study of antigens active in the tannic acid hemagglutination test present in filtrates of culture of Mycobacterium tuberculosis. J Immunol. 1955 Jul;75(1):15–21. [PubMed] [Google Scholar]
  3. BOYDEN S. V. The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J Exp Med. 1951 Feb;93(2):107–120. doi: 10.1084/jem.93.2.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CHRISTIAN C. L. Characterization of the reactant (gamma globulin factor) in the F II precipitin reaction and the F II tanned sheep cell agglutination test. J Exp Med. 1958 Jul 1;108(1):139–157. doi: 10.1084/jem.108.1.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Das P. C., Allan A. G., Woodfield D. G., Cash J. D. Fibrin degradation products in sera of normal subjects. Br Med J. 1967 Dec 23;4(5581):718–720. doi: 10.1136/bmj.4.5581.718. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. EPSTEIN W. V., GROSS D. NATURALLY OCCURRING HUMAN ANTIBODY REACTING WITH BENCE JONES PROTEINS. J Exp Med. 1964 Nov 1;120:733–745. doi: 10.1084/jem.120.5.733. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FERREIRA H. C., SILVA M. P., MURAT L. G., FERRAZ C. N. FIBRINOGEN-POOR PLATELETS AS ONE OF THE DEFECTS IN GLANZMANN PURPURA. Rev Hosp Clin Fac Med Sao Paulo. 1964 Nov-Dec;19:293–300. [PubMed] [Google Scholar]
  8. FISHER S. The estimation in vitro of small amounts of diphtheria antitoxin by means of a haemagglutination technique. J Hyg (Lond) 1952 Dec;50(4):445–456. doi: 10.1017/s0022172400019732. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FULTHORPE A. J. Factors influencing haemagglutination tests with tetanux antitoxin. Immunology. 1959 Apr;2(2):104–111. [PMC free article] [PubMed] [Google Scholar]
  10. Fox F. J., Jr, Wide L., Killander J., Gemzell C. Determination of fibrinogen and fibrinolysis products in human plasma by an immunochemical method. Scand J Clin Lab Invest. 1965;17(4):341–348. doi: 10.3109/00365516509077061. [DOI] [PubMed] [Google Scholar]
  11. GEORGE M., VAUGHAN J. H. Observations on the nature of the antigen in tanned red cell hemagglutination. J Immunol. 1962 Feb;88:191–198. [PubMed] [Google Scholar]
  12. HELLER G., JACOBSON A. S., KOLODNY M. H., KAMMERER W. H. The hemagglutination test for rheumatoid arthritis. II. The influence of human plasma fraction II (gamma globulin) on the reaction. J Immunol. 1954 Jan;72(1):66–78. [PubMed] [Google Scholar]
  13. Herbert W. J. A study of factors influencing the sensitivity to antibody of tanned, ovalbumin-coated, formalinized sheep red cells. Immunology. 1967 Nov;13(5):459–468. [PMC free article] [PubMed] [Google Scholar]
  14. LINZ R., LECOCQ E., MANDELBAUM E. [The adsorption of antigens on tanned erythrocytes]. Ann Inst Pasteur (Paris) 1961 Sep;101:367–381. [PubMed] [Google Scholar]
  15. MAURER P. H., GERULAT B. F., PINCHUCK P. ANTIGENICITY OF POLYPEPTIDES (POLY-ALPHA-AMINO ACIDS). V. STUDIES IN RABBITS. J Immunol. 1963 Mar;90:381–387. [PubMed] [Google Scholar]
  16. Mackay A. V., Das P. C., Myerscough P. R., Cash J. D. Fibrinolytic components of human uterine arterial and venous blood. J Clin Pathol. 1967 May;20(3):227–230. doi: 10.1136/jcp.20.3.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Merskey C., Kleiner G. J., Johnson A. J. Quantitative estimation of split products of fibrinogen in human serum, relation to diagnosis and treatment. Blood. 1966 Jul;28(1):1–18. [PubMed] [Google Scholar]
  18. SHIOIRI K. THE OBSERVATION OF PROTEINS ADSORBED ON ERYTHROCYTES TREATED WITH TANNIC ACID IN PASSIVE HEMAGGLUTINATION TESTS (BOYDEN'S METHOD). Jpn J Exp Med. 1964 Dec;34:345–359. [PubMed] [Google Scholar]
  19. STAVITSKY A. B. Micromethods for the study of proteins and antibodies. I. Procedure and general applications of hemagglutination and hemagglutination-inhibition reactions with tannic acid and protein-treated red blood cells. J Immunol. 1954 May;72(5):360–367. [PubMed] [Google Scholar]
  20. STEFFEN C., ROSAK M. IN VITRO DEMONSTRATION OF ANTI-OVALBUMIN SPECIFICITY OF LYMPH NODE CELLS IN DELAYED TYPE HYPERSENSITIVITY. J Immunol. 1963 Mar;90:337–346. [PubMed] [Google Scholar]
  21. Woodfield D. G., Cole S. K., Allan A. G., Cash J. D. Serum fibrin degradation products throughout normal pregnancy. Br Med J. 1968 Dec 14;4(5632):665–668. doi: 10.1136/bmj.4.5632.665. [DOI] [PMC free article] [PubMed] [Google Scholar]

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