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. 2015 Aug 22;6(30):29599–29613. doi: 10.18632/oncotarget.5011

Figure 5. RAD6 and ASF1A negatively correlated in tumor cell proliferation and migration.

Figure 5

A. Human lung cancer H1299 cells were transfected with an GFP empty vector, or a RAD6A-GFP, or a ASF1A-Red, or a combination of these two plasmids, and stable cell lines were generated. The cells were then used for a soft agar assay to determine the ability of cell proliferation. The quantification of the soft agar data are shown on the right. B. Knockdown of RAD6 expression promotes liver cell migration in an ASF1A-dependent manner. HL-7702 cells were transfected with or without RAD6A and RAD6B-specific siRNAs together with or without an ASF1A-specific siRNA as indicated for 48 h. The cells were then used for a Transwell assay to determine the cell migration ability. The quantification of the Transwell data and related RNAi efficiency are shown below.