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. 2015 Aug 20;6(30):29847–29859. doi: 10.18632/oncotarget.4913

Figure 1. NDRG1 binds to GSK-3β and Nur77 in HepG2 and Huh7 cells.

Figure 1

A. SDS-PAGE gel analysis of purified NDRG1 (indicated by arrow). B. Pull-down assay demonstrating the interaction between NDRG1 and GSK-3β, and between NDRG1 and Nur77 in Huh7 and HepG2 cells with endogenous levels of NDRG1, but not in Hep3B cells, which lacks NDRG1 expression under normoxia. C. Immunofluorescence staining showing the co-localization of NDRG1 with GSK-3β or with Nur77 in Huh7 and HepG2 cells, but not in Hep3B cells under normoxia. DAPI was used to stain the nucleus (400× magnification; scale bars: 5 μm).