Figure 3. p53-dependent repression of Survivin (BIRC5), CDC25C, and PLK1 is mediated via CDE/CHR elements but not through CCAAT-boxes or p53 binding sites.

Luciferase reporter assays from wild-type (wt) or mutant Survivin, CDC25C, and PLK1 promoter constructs transfected into HCT116 p53^−/− cells. Mutants of the potential transcription factor binding sites CDE, CHR, CDE/CHR (CDE and CHR mutated), p53BS, or deletions of CCAAT-boxes were tested. Plasmid constructs were cotransfected in HCT116 p53^−/− cells with p53R175H mutant (p53 mut) or p53 wild-type (p53 wt) expression vectors. The pGL4.10 luciferase reporter vector was used as negative control. Relative luciferase units (RLU) are shown.