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. 2015 Nov 2;6(39):41479–41496. doi: 10.18632/oncotarget.6286

Figure 2. Effect of PA and MA in hepatocyte lipoapoptosis.

Figure 2

PMH were treated with PA, MA and combination PA plus MA (PM) and cells were examine by phase contrast and staining with propidium iodide (PI) or Hoëscht A. Alternatively, cell death caused by PA and MA was determined by trypan blue exclusion to determine percentage of cell death B. and C. In some cases, extracts were processed for the determination of PARP processing and caspase 3 cleavage D., as well as cytochrome c release E. The effect of pan-caspase inhibitor z-VAD-Fmk (15μM) on PA and PA plus MA (PM) was determined F. The Results are the mean±SEM of N = 6-8 individual experiments. *p < 0.05 vs. control and PA-treated PMH.