Skip to main content
. 2015 Nov 22;6(39):41497–41507. doi: 10.18632/oncotarget.6359

Figure 2. Donor-derived stem cells express EMT markers in squamous cell carcinoma.

Figure 2

A. Combined CD133 (green) and Ki67 (red) immunofluorescent stainings show CD133 expressing cells in SCC invasive areas. These cells do not co-express Ki67 (bar=100μm). B. Immunoperoxydase staining of vimentin within cells of SCC basal layer (bar=100μm, higher magnification bar=25μm). C. Combined CD133 (green) and vimentin (red) immunofluorescence stainings show double positive cells (arrow heads) in SCC outer cell layers (bar=25μm). D. In SCCs of the three kidney-transplant recipients studied, CDH1 (E-cadherin) is under-expressed in cells co-expressing CD133 and vimentin compared with cells only expressing CD133, and with normal keratinocytes. E. In the same patients, SNAI1 (Snail1) is overexpressed in cells co-expressing CD133 and vimentin compared with cells only expressing CD133, and with normal keratinocytes. F. Immunoperoxydase staining of Snail-Slug within cells of SCC basal layer and invasive areas (bar= 150μm). G. Combined CD133 (green) and Snail-Slug (red) immunofluorescent stainings show CD133 expressing cells in SCC outer cell layers. (bar=25μm). H. In SCCs of the three kidney-transplant recipients studied, cells co-expressing CD133 and vimentin have more Y-chromosome detected by droplet digital PCR than cells only expressing CD133 (p<0.05, Chi-square test). I. In SCCs of the three kidney-transplant recipients studied, cells co-expressing CD133 and Snail-Slug have more Y-chromosome detected by droplet digital PCR than cells only expressing CD133 (p<0.05, Chi-square test).