Figure 6. Dietary feeding of FKA induces apoptosis, inhibits cell proliferation and reduces Cullin1 NEDDylation and expression of Skp2 and p27 expression in vivo in the prostate of TRAMP mice.

A. A representative immunohistochemistry staining for TUNEL, Ki67, Skp2, P27 and NEDD8 in the prostates of 24-weeks-old, vehicle control versus 0.6% FKA diet fed TRAMP mice were shown. 200 x magnifications of images are presented. For TUNEL assay, 20 fields from ten mouse prostates were counted in each group, and the average number of positive stained cells per field is shown. For other staining, 12 fields were counted from eight mouse prostates in each group, and the percentage of positive stained cells was shown. Error bars were standard deviations. B. Mouse dorsolateral prostates (n=3 in vehicle control and 0.3% FKA diet groups, none of the samples has neuroendocrine carcinomas) were homogenized in RIPA buffer, indicated protein expressions were examined by Western blot analysis. Tubulin was used as a loading control and SV40 expression shows no difference in transgene expression between control and FKA fed groups.