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. 2015 Nov 14;6(38):40405–40417. doi: 10.18632/oncotarget.6334

Figure 2. Oncogenic Ras-induced macropinocytosis contributes to cellular growth under nutrient deficiency.

Figure 2

Figure 2

A. In MEFs expressing KRas G12V or HRas G12V, the reduction in cell growth following glutamine deprivation is reversed by the treatment with 2% BSA. Cell growth after 6 days of glutamine (Q) deprivation with or without 2% BSA was quantified by cell counting. Furthermore, cell numbers after treatment with 25 μM EIPA to inhibit macropinocytic BSA uptake during conditions of starvation supplemented with 2% BSA were counted. Relative growth rate is presented after normalization by number of cells under each starvation condition without BSA. B. In the human cancer lines harboring mutant KRas (MIA PaCa-2 or HCT116), supplementing with 2% BSA reversed the reduction in growth resulting from glutamine deprivation. C. In response to essential amino acid (EAA) deprivation, growth of MEFs expressing KRas G12V, MiaPaCa2 and HCT116 cells was restored by treatment with 2% BSA. Data are presented relative to the values observed in the glutamine or EAA withdrawal conditions. Error bars indicate mean +/− SEM for n = 3 independent experiments. Statistical significance was determined via a student's t-test.