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. 2015 Oct 20;6(38):40799–40814. doi: 10.18632/oncotarget.5695

Figure 1. miR-29b is differentially expressed in human ovarian cancers & normal ovaries, and involved in regulating ovarian cancer cell metabolism.

Figure 1

A. Normal ovarian epithelial cells HOSEpiC shows highest endogenous miR-29b expression compared with 4 selected ovarian cancer cell lines; B. qPCR results shows that miR-29b in benign ovarian epithelia is much higher than those in human cancerous ovarian epithelia; C. MTT assay results reveal that miR-29b mimics transfection led to a decrease in absorbance (OD value) at 570 nm, while miR-29b inhibitors transfection led to an increase in absorbance (OD value) at 570 nm in both A2780 and SKOV3 cells; D. qPCR results indicate that miR-29b expression varies in different metabolic conditions in ovarian cancer cell lines A2780 and SKOV3. E. and F. Inhibition of miR-29b expression by target inhibitors transfection leads to increased glucose consumption and lactate production in A2780 cells, and over-expression of miR-29b by target mimics transfection causes decreased glucose consumption and lactate production in SKOV3 cells. Data are presented as means ± SEM, n = 3. *p < 0.05 versus control.