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. 2015 Oct 16;6(38):41018–41032. doi: 10.18632/oncotarget.5879

Figure 1. Mutant p53 activates the SREBP-mediated signaling pathways in PCa cells.

Figure 1

A. Western blot analysis of p53, SREBP-1, SREBP-2, FASN and HMGCR expression in PC-3 cells stably transfected with various mutant p53s (V143A, R248W, R175H and R273H) or empty vector (EV). GAPDH was used as a loading control. P and N denote the precursor and nuclear forms of SREBPs, respectively. B. qPCR (left panel) and Western blot (right panel) analyses of p53, SREBP-1, SREBP-2, FASN and HMCCR expression in DU145 cells infected with p53-targeting shRNAs (shp53) or scrambled shRNA (shCon) lentivirus particles. qPCR data were normalized to β-actin and represent the mean ± SD of three independent triplicate experiments. *P < 0.05, **P < 0.01. GAPDH was used as a loading control for Western blot analysis. C. Western blot analysis of p53, SREBP-1, SREBP-2, FASN and HMCCR expression in PC-3 cells transiently transfected with wild-type p53 plasmid (WT TP53) or empty vector (EV). GAPDH was used as a loading control. P and N denote the precursor and nuclear forms of SREBPs, respectively.