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. 2015 Oct 15;6(38):41324–41338. doi: 10.18632/oncotarget.5847

Figure 1. Suppression of DR5, but not DR4, expression increases cancer cell invasion.

Figure 1

A–D. The indicated cell lines, in which DR5 was stably knocked down (A), knocked out (B), knocked down and then re-expressed (C) or knocked out and then re-expressed (D) as evaluated with Western blotting were plated in Matrigel invasion chambers for cell invasion assay and in 96-well plates for cell number estimation with MTS assay after approximately 48 h incubation. E. A549 cells transiently transfected with control (Ctrl), DR5 or DR4 siRNA were plated in 12-well plates for evaluation of DR5 or DR4 expression by Western blotting, in the Matrigel invasion chambers for invasion assay and in 96-well plates for cell number estimation after approximately 48 h incubation. The data are means ± SDs of triplicate (invasion) or quadruplicate (cell growth) determinations. WT, wild-type.