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. 2015 Oct 15;6(38):41324–41338. doi: 10.18632/oncotarget.5847

Figure 5. DR5 knockdown leads to ERK-, JNK-, c-Jun- and Fra-1-dependent MMP1 elevation (A and B) and enhanced cell invasion (C).

Figure 5

A. and B. A549 cells were transfected with the indicated siRNAs alone or in combinations and after 48 h, were harvested for preparation of whole-cell protein lysates and subsequent Western blot analysis. C. A549 cells transfected with the indicated siRNAs were seeded in 12-well plates for Western blotting, in Matrigel invasion chambers for cell invasion assay and in 96-well plates for cell growth measurements after approximately 48 h incubation. The data are means ± SDs of duplicate (invasion) or quadruplicate (cell growth) determinations.