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Mean area under the curve ± SEM of the relative responses at 04:00, 08:00, 16:00 and 20:00 hrs in n=16 normal male Long Evans rats (Friedman’s test P=0.69) ( A). Using the same data set but now depicting per volume relative responses on different time points (Friedman’s test P=0.55, 0.95 and 0.93; B, C and D respectively).
