Figure 7. Summary of RNAi and mutant experiments for female CHCs.
UAS-RNAi target gene and the CG10097e00276 mutant are indicated on the horizontal axis. CHC names and numbers are listed on the y-axis. Data are color coded to represent P-values (P ≤ 0.05) from t-tests for the mean differences of the experimental and the control lines. Black = no significant change; blue = significant decrease; green = significant increase; gray = not applicable (peaks 46 and 57 split into two peaks for the CG10097 mutant).
Figure 7—figure supplement 1. Functional validation PCA and total CHCs for RNAi-app.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 2. Functional validation PCA and total CHCs for RNAi-CG5599.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 3. Functional validation PCA and total CHCs for RNAi-CG7724.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 4. Functional validation PCA and total CHCs for RNAi-CG8680.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 5. Functional validation PCA and total CHCs for RNAi-CG8814.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 6. Functional validation PCA and total CHCs for RNAi-CG9458.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 7. Functional validation PCA and total CHCs for RNAi-CG9801.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 8. Functional validation PCA and total CHCs for mutant CG10097.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 9. Functional validation PCA and total CHCs for RNAi-CG13091.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 10. Functional validation PCA and total CHCs for RNAi-CG14688.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 11. Functional validation PCA and total CHCs for RNAi-CG16979.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 12. Functional validation PCA and total CHCs for RNAi-CG18609.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 13. Functional validation PCA and total CHCs for RNAi-CG30008.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 14. Functional validation PCA and total CHCs for RNAi-Cyp4s3.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 15. Functional validation PCA and total CHCs for RNAi-Cyp9f2.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 16. Functional validation PCA and total CHCs for RNAi-Cyp49a1.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 17. Functional validation PCA and total CHCs for RNAi-Desi.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 18. Functional validation PCA and total CHCs for RNAi-Irc.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 19. Functional validation PCA and total CHCs for RNAi-Lip2.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 20. Functional validation PCA and total CHCs for RNAi-Nrt.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 21. Functional validation PCA and total CHCs for RNAi-PHGPx.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 22. Functional validation PCA and total CHCs for RNAi-Prx6005.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 23. Functional validation PCA and total CHCs for RNAi-Pxd.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.
Figure 7—figure supplement 24. Functional validation PCA and total CHCs for RNAi-pxn.
(A) PCA biplots for females and males, ○ = female, ○ = male, and ● = control samples. (B) PC1 and PC2 eigenvectors. (C) Box-plots of female and male total amount of CHCs (µg/fly). P-values are reported for the Satterthwaite test, *: P < 0.05, **: P < 0.01, ***: P < 0.001.