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. 1974 Jul;27(7):548–557. doi: 10.1136/jcp.27.7.548

Intracellular immunoglobulins

A comparative study on three standard tissue processing methods using horseradish peroxidase and fluorochrome conjugates

J Burns 1, Maria Hambridge 1, C R Taylor 1
PMCID: PMC475394  PMID: 4607520

Abstract

Formalin fixation followed by routine paraffin embedding allows the demonstration of intracellular immunoglobulin by a sandwich technique using either peroxidase or fluorescein isothyocyanate labelled antibody conjugates. The results compared favourably with those obtained using either the fresh frozen or the Sainte-Marie method for preserving tissue immunoglobulins. The formalin-paraffin method with peroxidase-labelled antibody has advantages for routine use. Morphology is excellent, preparations are permanent, and retrospective studies of stored paraffin-embedded tissue are possible.

Some of the problems of labelled antibody studies are discussed.

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These references are in PubMed. This may not be the complete list of references from this article.

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