Abstract
A simple, cheap and readily available test system for biological safety cabinets is described. It depends on the containment of an aerosol of Bacillus subtilis spores generated in a BIRD micronebulizer and the measurement of air flows with an anemometer. The system was set up to survey new equipment but equally valuable results have been obtained from tests during use. New units were often badly installed and used equipment was poorly maintained. It is suggested that any department which has a need for a biological safety cabinet must be in a position to test its function.
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- Barbeito M. S., Taylor L. A. Containment of microbial aerosols in a microbiological safety cabinet. Appl Microbiol. 1968 Aug;16(8):1225–1229. doi: 10.1128/am.16.8.1225-1229.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Beeby M. M., Whitehouse C. E. A bacterial spore test piece for the control of ethylene oxide sterilization. J Appl Bacteriol. 1965 Dec;28(3):349–360. doi: 10.1111/j.1365-2672.1965.tb02163.x. [DOI] [PubMed] [Google Scholar]
- Hayes B., Robinson J. S. An assessment of methods of humidification of inspired gas. Br J Anaesth. 1970 Feb;42(2):94–104. doi: 10.1093/bja/42.2.94-a. [DOI] [PubMed] [Google Scholar]
- Kenny M. T., Sabel F. L. Particle size distribution of Serratia marcescens aerosols created during common laboratory procedures and simulated laboratory accidents. Appl Microbiol. 1968 Aug;16(8):1146–1150. doi: 10.1128/am.16.8.1146-1150.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Line S. J. Safety of portable inoculation cabinets. J Clin Pathol. 1972 Jan;25(1):93–94. doi: 10.1136/jcp.25.1.93. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MAY K. R., HARPER G. J. The efficiency of various liquid impinger samplers in bacterial aerosols. Br J Ind Med. 1957 Oct;14(4):287–297. doi: 10.1136/oem.14.4.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REITMAN M., WEDUM A. G. Microbiological safety. Public Health Rep. 1956 Jul;71(7):659–665. [PMC free article] [PubMed] [Google Scholar]
- WEDUM A. G. LABORATORY SAFETY IN RESEARCH WITH INFECTIOUS AEROSOLS. Public Health Rep. 1964 Jul;79:619–633. [PMC free article] [PubMed] [Google Scholar]
- WILLIAMS R. E., LIDWELL O. M. A protective cabinet for handling infective material in the laboratory. J Clin Pathol. 1957 Nov;10(4):400–402. doi: 10.1136/jcp.10.4.400. [DOI] [PMC free article] [PubMed] [Google Scholar]