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. 1993 Oct 15;90(20):9235–9236. doi: 10.1073/pnas.90.20.9235

Functional analysis suggests unexpected role for conserved active-site residue in enzyme of known structure.

P Schimmell 1
PMCID: PMC47541  PMID: 8415683

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chung H. H., Benson D. R., Schultz P. G. Probing the structure and mechanism of Ras protein with an expanded genetic code. Science. 1993 Feb 5;259(5096):806–809. doi: 10.1126/science.8430333. [DOI] [PubMed] [Google Scholar]
  2. Climie S., Ruiz-Perez L., Gonzalez-Pacanowska D., Prapunwattana P., Cho S. W., Stroud R., Santi D. V. Saturation site-directed mutagenesis of thymidylate synthase. J Biol Chem. 1990 Nov 5;265(31):18776–18779. [PubMed] [Google Scholar]
  3. Hardy L. W., Finer-Moore J. S., Montfort W. R., Jones M. O., Santi D. V., Stroud R. M. Atomic structure of thymidylate synthase: target for rational drug design. Science. 1987 Jan 23;235(4787):448–455. doi: 10.1126/science.3099389. [DOI] [PubMed] [Google Scholar]
  4. Hardy L. W., Nalivaika E. Asn177 in Escherichia coli thymidylate synthase is a major determinant of pyrimidine specificity. Proc Natl Acad Sci U S A. 1992 Oct 15;89(20):9725–9729. doi: 10.1073/pnas.89.20.9725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Liu L., Santi D. V. Asparagine 229 in thymidylate synthase contributes to, but is not essential for, catalysis. Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8604–8608. doi: 10.1073/pnas.90.18.8604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Liu L., Santi D. V. Exclusion of 2'-deoxycytidine 5'-monophosphate by asparagine 229 of thymidylate synthase. Biochemistry. 1993 Sep 14;32(36):9263–9267. doi: 10.1021/bi00087a001. [DOI] [PubMed] [Google Scholar]
  7. Liu L., Santi D. V. Mutation of asparagine 229 to aspartate in thymidylate synthase converts the enzyme to a deoxycytidylate methylase. Biochemistry. 1992 Jun 9;31(22):5100–5104. doi: 10.1021/bi00137a002. [DOI] [PubMed] [Google Scholar]
  8. Matthews D. A., Appelt K., Oatley S. J., Xuong N. H. Crystal structure of Escherichia coli thymidylate synthase containing bound 5-fluoro-2'-deoxyuridylate and 10-propargyl-5,8-dideazafolate. J Mol Biol. 1990 Aug 20;214(4):923–936. doi: 10.1016/0022-2836(90)90346-N. [DOI] [PubMed] [Google Scholar]
  9. Montfort W. R., Perry K. M., Fauman E. B., Finer-Moore J. S., Maley G. F., Hardy L., Maley F., Stroud R. M. Structure, multiple site binding, and segmental accommodation in thymidylate synthase on binding dUMP and an anti-folate. Biochemistry. 1990 Jul 31;29(30):6964–6977. doi: 10.1021/bi00482a004. [DOI] [PubMed] [Google Scholar]
  10. Perry K. M., Fauman E. B., Finer-Moore J. S., Montfort W. R., Maley G. F., Maley F., Stroud R. M. Plastic adaptation toward mutations in proteins: structural comparison of thymidylate synthases. Proteins. 1990;8(4):315–333. doi: 10.1002/prot.340080406. [DOI] [PubMed] [Google Scholar]
  11. Shoichet B. K., Stroud R. M., Santi D. V., Kuntz I. D., Perry K. M. Structure-based discovery of inhibitors of thymidylate synthase. Science. 1993 Mar 5;259(5100):1445–1450. doi: 10.1126/science.8451640. [DOI] [PubMed] [Google Scholar]

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