Abstract
A comparison has been made of Lowenstein-Jensen medium, with or without added pyruvate, 7H11 oleic acid-albumin agar slopes made selective by the addition of antibiotics, and selective Kirchner medium in the isolation of tubercle bacilli from specimens other than sputum. A combination of a standard mild preliminary treatment with sulphuric acid and selective medium reduced the contamination rate to 0·1%. Neither acid treatment nor antibiotics appeared to reduce the isolation rate. An effective procedure is to treat all specimens (except cerebrospinal fluid) with acid and culture on two Lowenstein-Jensen slopes (one with pyruvate), a 7H11 selective slope, and in two bottles of selective Kirchner medium.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cavanagh P., Keyes A. B. The use of pyruvate-containing egg medium in the isolation of Sudanese stains of tubercle bacilli. Tubercle. 1968 Sep;49(3):325–327. doi: 10.1016/0041-3879(68)90054-8. [DOI] [PubMed] [Google Scholar]
- Hughes M. H. Pyruvic acid egg medium for tubercle bacilli. J Clin Pathol. 1966 Jan;19(1):73–75. doi: 10.1136/jcp.19.1.73. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Liu P. I., McGregor D. H., Faucher I., Jinks W. L., Miller L. A., Green L., Liu J. G. Comparison of three culture media for isolation of Mycobacterium tuberculosis: a 6-year study. Appl Microbiol. 1973 Dec;26(6):880–883. doi: 10.1128/am.26.6.880-883.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lorian V. Comparison of mycobacterial cultures on agar and Lowenstein medium. Appl Microbiol. 1967 Sep;15(5):1202–1205. doi: 10.1128/am.15.5.1202-1205.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MARKS J. PYRUVIC ACID IN THE CULTIVATION OF TUBERCLE BACILLI. Mon Bull Minist Health Public Health Lab Serv. 1963 Sep;22:150–152. [PubMed] [Google Scholar]
- Marks J. Ending the routine guinea-pig test. Tubercle. 1972 Mar;53(1):31–34. doi: 10.1016/0041-3879(72)90043-8. [DOI] [PubMed] [Google Scholar]
- Mitchison D. A., Allen B. W., Carrol L., Dickinson J. M., Aber V. R. A selective oleic acid albumin agar medium for tubercle bacilli. J Med Microbiol. 1972 May;5(2):165–175. doi: 10.1099/00222615-5-2-165. [DOI] [PubMed] [Google Scholar]
- Mitchison D. A., Allen B. W., Lambert R. A. Selective media in the isolation of tubercle bacilli from tissues. J Clin Pathol. 1973 Apr;26(4):250–252. doi: 10.1136/jcp.26.4.250. [DOI] [PMC free article] [PubMed] [Google Scholar]