Abstract
A technique of protein electrophoresis suitable for routine use which ensures good separation of the prealbumin fraction is described. The clinical value of visually estimating this protein fraction from the electrophoretic strip is shown with reference to 134 cases. Low levels of prealbumin were found to be associated with liver disorders, malignancy, collagen diseases, congestive cardiac failure, and burns. Finally, pitfalls in electrophoretic technique which may cause failure to visualize the prealbumin fraction are discussed.
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- ALY F. W., NIEDERHELLMANN K. H. Untersuchungen zum Präalbumin im menschlichen Serum. I. Methodische Angaben zum papierelektrophoretischen Nachweis des Präalbumins auf Filterpapier; quantitative Veränderungen dés Präalbumins. Klin Wochenschr. 1958 Oct 15;36(20):954–959. doi: 10.1007/BF01486701. [DOI] [PubMed] [Google Scholar]
- Agostoni A., Marasini B., Stabilini R., Del Ninno E., Pontello M. Multivariate analysis of serum protein assays in chronic hepatitis and postnecrotic cirrhosis. Clin Chem. 1974 Apr;20(4):428–429. [PubMed] [Google Scholar]
- INGBAR S. H. Pre-albumin: a thyroxinebinding protein of human plasma. Endocrinology. 1958 Aug;63(2):256–259. doi: 10.1210/endo-63-2-256. [DOI] [PubMed] [Google Scholar]
- Kohn J. A multi-sample applicator for zone electrophoresis. Clin Chim Acta. 1967 Oct;18(1):65–68. doi: 10.1016/0009-8981(67)90247-1. [DOI] [PubMed] [Google Scholar]
- OPPEHNEIMER J. H., SURKS M. I., BERNSTEIN G., SMITY J. C. METABOLISM OF IODINE-131--LABELED THYROXINE-BINDING PREALBUMIN IN MAN. Science. 1965 Aug 13;149(3685):748–750. doi: 10.1126/science.149.3685.748. [DOI] [PubMed] [Google Scholar]
- SCHONENBERGER M., SCHULTZE H. E., SCHWICK G. Uber ein Präalbumin des menschlichen Serums. Biochem Z. 1956;328(4):267–284. [PubMed] [Google Scholar]