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. 1993 Oct 15;90(20):9384–9387. doi: 10.1073/pnas.90.20.9384

Transnuclear retrotransposition of the Tad element of Neurospora.

J A Kinsey 1
PMCID: PMC47572  PMID: 8415711

Abstract

Tad is a LINE-like DNA element found in Neutrospora crassa. A Neurospora artificial intron based on the first intron of the am (glutamate dehydrogenase) gene was constructed and introduced, in the correct orientation, into a unique Nru I site in open reading frame 1 of an active Tad element, Tad1-1. Transformants containing the Tad element with the artificial intron were placed in forced heterokaryons with strains lacking Tad elements. Tad was shown to transpose between nuclei in these heterokaryons. Examination of the transposed Tad elements showed that the intron had been precisely removed in all cases. This confirms that Tad is a retrotransposon and that there is a cytoplasmic phase in these retrotransposition events.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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