Abstract
A comparison has been made of the efficiency of human embryo kidney (HEK) cills, HeLa cells, and WI38 cells for the isolation of viruses from the eyes of patients suffering from acute conjunctivitis or keratoconjunctivitis. From a total of 99 specimens 21 adenoviruses (serotypes 3, 4, 7, 8, and 13) were isolated in HEK cells, eight (serotypes 3 and 8) in HeLa cells, and four (serotype 3) in WI38 cells. Of the ten herpes simplex viruses isolated nine were recovered in HEK cells, seven in WI38 cells, and none in HeLa cells. The combination of HeLa cells and WI38 cells is not considered an adequate alternative to the difficult-to-obtain HEK cells for the isolation of viruses from the eye.
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Selected References
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- BEALE A. J., DOANE F., ORMSBY H. L. Studies on adenorivus infections of the eye in Toronto. Am J Ophthalmol. 1957 Apr;43(4 Pt 2):26–31. doi: 10.1016/0002-9394(57)91471-x. [DOI] [PubMed] [Google Scholar]
- Bell E. J., Martin K. W., Ross A. C. Laboratory diagnosis of epidemic keratoconjunctivitis. J Med Microbiol. 1969 May;2(2):125–130. doi: 10.1099/00222615-2-2-125. [DOI] [PubMed] [Google Scholar]
- Darougar S., Jones B. R., Kinnison J. R., Vaughan-Jackson J. D., Dunlop E. M. Chlamydial infection. Advances in the diagnostic isolation of Chlamydia, including TRIC agent, from the eye, genital tract, and rectum. Br J Vener Dis. 1972 Dec;48(6):416–420. doi: 10.1136/sti.48.6.416. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ellison E. D., Kaufman H. E., Little J. M. Comparison of methods for the laboratory diagnosis of ocular adenovirus type 3 infection. Invest Ophthalmol. 1969 Oct;8(5):484–487. [PubMed] [Google Scholar]
- Gardner P. S., McQuillin J., Black M. M., Richardson J. Rapid diagnosis of herpesvirus hominis infections in superficial lesions by immunofluorescent antibody technics. Br Med J. 1968 Oct 12;4(5623):89–92. doi: 10.1136/bmj.4.5623.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HANNA L., JAWETZ E., COLEMAN V. R. Studies on herpes simplex. VIII. The significance of isolating herpes-simplex virus from the eye. Am J Ophthalmol. 1957 Apr;43(4 Pt 2):126–131. [PubMed] [Google Scholar]
- HART D. R., BRIGHTMAN V. J., READSHAW G. G., PORTER G. T., TULLY M. J. TREATMENT OF HUMAN HERPES SIMPLEX KERATITIS WITH IDOXURIDINE. Arch Ophthalmol. 1965 May;73:623–634. doi: 10.1001/archopht.1965.00970030625006. [DOI] [PubMed] [Google Scholar]
- JONES B. R., ANDREWS B. E., HENDERSON W. G., SCHOFIELD P. B. The pattern of conjunctivitis at Moorfields during 1956. Trans Opthal Soc U K. 1957;77:291–305. [PubMed] [Google Scholar]
- SOMMERVILLE R. G. Epidemic keratoconjunctivitis; an adenovirus infection. J Hyg (Lond) 1958 Mar;56(1):101–107. doi: 10.1017/s0022172400037591. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WARD T. G., HUEBNER R. J., ROWE W. P., RYAN R. W., BELL J. A. Production of pharyngoconjunctival fever in human volunteers inoculated with APC viruses. Science. 1955 Dec 2;122(3179):1086–1087. doi: 10.1126/science.122.3179.1086. [DOI] [PubMed] [Google Scholar]
- Wegman D. H., Guinee V. F., Milian S. J. Epidemic keratoconjunctivitis. Am J Public Health Nations Health. 1970 Jul;60(7):1230–1237. doi: 10.2105/ajph.60.7.1230. [DOI] [PMC free article] [PubMed] [Google Scholar]
- White D. O., Shew M. A., Howsam K. G., Robertson I. F. Herpes simplex virus infection of the cornea. Med J Aust. 1968 Jul 13;2(2):59–63. doi: 10.5694/j.1326-5377.1968.tb29290.x. [DOI] [PubMed] [Google Scholar]