Abstract
Cultured lymph node cell supernatants from five out of six cases of Hodgkin's disease were relatively more chemotactic for peripheral blood eosinophils than neutrophils. In contrast, supernatants from two cases of lymphocytic lymphoma and four nodes showing reactive hyperplasia were chemotactic for neutrophils but had little eosinophil chemotactic activity. In most instances the dgree of eosinophil infiltration observed histologically in the Hodgkin's lymph node correlated with elaboration of eosinophil chemotactic activity from the cultured cells. Following gel-filtration of three of the Hodgkin's lymphnode supernatants, four peaks of eosinophil chemotactic activity were demonstrated in each case. One of these corresponded in molecular size to th previously described eosinophil chemotactic factor of anaphylaxis (ECF-A). It is suggested that the eosinophil chemotactic activity of cultured lymph node cell supernatants may be on value in the diagnosis and classification of Hodgkin's disease.
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Selected References
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- Altman L. C., Snyderman R., Oppenheim J. J., Mergenhagen S. E. A human mononuclear leukocyte chemotactic factor: characterization, specificity and kinetics of production by homologous leukocytes. J Immunol. 1973 Mar;110(3):801–810. [PubMed] [Google Scholar]
- Cohen S., Ward P. A. In vitro and in vivo activity of a lymphocyte and immune complex-dependent chemotactic factor for eosinophils. J Exp Med. 1971 Jan 1;133(1):133–146. doi: 10.1084/jem.133.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dorfman R. F. Biology of malignant neoplasia of the lymphoreticular tissues. J Reticuloendothel Soc. 1972 Sep;12(3):239–256. [PubMed] [Google Scholar]
- Habeshaw J. A. A serum-protein-free medium for the culture of macrophages and related cells. J Pathol. 1972 Sep;108(1):95–96. doi: 10.1002/path.1711080112. [DOI] [PubMed] [Google Scholar]
- Kay A. B., Austen K. F. The IgE-mediated release of an eosinophil leukocyte chemotactic factor from human lung. J Immunol. 1971 Sep;107(3):899–902. [PubMed] [Google Scholar]
- Kay A. B., Stechschulte D. J., Austen K. F. An eosinophil leukocyte chemotactic factor of anaphylaxis. J Exp Med. 1971 Mar 1;133(3):602–619. doi: 10.1084/jem.133.3.602. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kay A. B. Studies on eosinophil leucocyte migration. II. Factors specifically chemotactic for eosinophils and neutrophils generated from guinea-pig serum by antigen-antibody complexes. Clin Exp Immunol. 1970 Nov;7(5):723–737. [PMC free article] [PubMed] [Google Scholar]
- Lukes R. J., Butler J. J. The pathology and nomenclature of Hodgkin's disease. Cancer Res. 1966 Jun;26(6):1063–1083. [PubMed] [Google Scholar]
- Smithers D. W. Hodgkin's disease. I. Br Med J. 1967 Apr 29;2(5547):263–268. doi: 10.1136/bmj.2.5547.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smithers D. W. Hodgkin's disease: one entity or two? Lancet. 1970 Dec 19;2(7686):1285–1287. doi: 10.1016/s0140-6736(70)92245-2. [DOI] [PubMed] [Google Scholar]
- Stuart A. E. Immunological aspects of reticulum cell neoplasia. Br Med J. 1970 Nov 14;4(5732):423–424. doi: 10.1136/bmj.4.5732.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vianna N. J., Greenwald P., Davies J. N. Nature of Hodgkin's disease agent. Lancet. 1971 Apr 10;1(7702):733–736. doi: 10.1016/s0140-6736(71)91995-7. [DOI] [PubMed] [Google Scholar]
- Ward P. A., Remold H. G., David J. R. Leukotactic factor produced by sensitized lymphocytes. Science. 1969 Mar 7;163(3871):1079–1081. doi: 10.1126/science.163.3871.1079. [DOI] [PubMed] [Google Scholar]
- Wasserman S. I., Goetzl E. J., Ellman L., Austen K. F. Tumor-associated eosinophilotactic factor. N Engl J Med. 1974 Feb 21;290(8):420–424. doi: 10.1056/NEJM197402212900802. [DOI] [PubMed] [Google Scholar]