Abstract
We have recently utilized a prototype model of the Beckman Enzyme Activity Analyzer System-TR in our laboratory measuring various serum enzyme activities which include: alkaline phosphatase (ALP), E.C.3.1.3.1; creatine kinase (CK), E.C.2.7.3.2; hydroxybutyrate dehydrogenase (HBD), E.C.1.1.1.30; lactate dehydrogenase (LD), E.C.1.1.1.27; aspartate transaminase (AST), E.C.2.6.1.1; and alanine transaminase (ALT), E.C.2.6.1.2. Precision was found to be good. Sample activities could be measured as high as 1000 IU/1. The carryover studies fell within 2 SD of the means of the enzyme control studies. Coefficients of variation for ALP and CK were in the ranges of 0-40-2-14% and 0-52-4-30%, respectively. Correlation studies were done with GemSAEC and Gilford 300 N Spectrophotometer and the results were accurate, precise, and reproducible.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- AMADOR E., DORFMAN L. E., WACKER W. E. SERUM LACTIC DEHYDROGENASE ACTIVITY: AN ANALYTICAL ASSESSMENT OF CURRENT ASSAYS. Clin Chem. 1963 Aug;12:391–399. [PubMed] [Google Scholar]
- AMADOR E., WACKER W. E. Serum glutamic-oxaloacetic transaminase activity. A new modification and an anaytical assessment of current assay technics. Clin Chem. 1962 Aug;8:343–350. [PubMed] [Google Scholar]
- Bowers G. N., Jr, McComb R. B. A continuous spectrophotometric method for measuring the activity of serum alkaline phosphatase. Clin Chem. 1966 Feb;12(2):70–89. [PubMed] [Google Scholar]
- Gay R. J., McComb R. B., Bowers G. N., Jr Optimum reaction conditions for human lactate dehydrogenase isoenzymes as they affect total lactate dehydrogenase activity. Clin Chem. 1968 Aug;14(8):740–753. [PubMed] [Google Scholar]
- HENRY R. J., CHIAMORI N., GOLUB O. J., BERKMAN S. Revised spectrophotometric methods for the determination of glutamic-oxalacetic transaminase, glutamic-pyruvic transaminase, and lactic acid dehydrogenase. Am J Clin Pathol. 1960 Oct;34:381–398. doi: 10.1093/ajcp/34.4_ts.381. [DOI] [PubMed] [Google Scholar]
- OLIVER I. T. A spectrophotometric method for the determination of creatine phosphokinase and myokinase. Biochem J. 1955 Sep;61(1):116–122. doi: 10.1042/bj0610116. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROSALKI S. B., WILKINSON J. H. Reduction of alpha-ketobutyrate by human serum. Nature. 1960 Dec 24;188:1110–1111. doi: 10.1038/1881110a0. [DOI] [PubMed] [Google Scholar]
- Rosalki S. B. An improved procedure for serum creatine phosphokinase determination. J Lab Clin Med. 1967 Apr;69(4):696–705. [PubMed] [Google Scholar]