Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1975 Aug;28(8):631–635. doi: 10.1136/jcp.28.8.631

Immunochemical studies of the endocrine cells of the gastrointestinal tract. II An immunoperoxide technique for the localization of secretin containing cells in human duodenum.

G Robinson, I Dawson
PMCID: PMC475791  PMID: 1102565

Abstract

Endocrine cells containing secretin have been identified in the epithelium lining human duodenum by direct and indirect immunoperoxidase techniques using immune sera raised against pur natural secretin. The techniques were applied to sections of carbodiimide-fixed tissue embedded in polyethylene glycol. Some sections, stained by a modified indirect technique, were processed for electron microscopy; secretin-containing granules were present by ultrastructural preservation was too poor to be of value. The potential advantages of a peroxidase technique over fluorescein-coniugated antisera are discussed.

Full text

PDF
631

Images in this article

633Image
on p.633
633Image
on p.633

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avrameas S., Ternynck T. Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration. Immunochemistry. 1971 Dec;8(12):1175–1179. doi: 10.1016/0019-2791(71)90395-8. [DOI] [PubMed] [Google Scholar]
  2. Bussolati G., Canese M. G. Electron microscopical identification of the immunofluorescent gastrin cells in the cat pyloric mucosa. Histochemie. 1972;29(3):198–206. doi: 10.1007/BF00306143. [DOI] [PubMed] [Google Scholar]
  3. Bussolati G., Capella C., Solcia E., Vassallo G., Vezzadini P. Ultrastructural and immunofluorescent investigations on the secretin cell in the dog intestinal mucosa. Histochemie. 1971;26(3):218–227. doi: 10.1007/BF00305655. [DOI] [PubMed] [Google Scholar]
  4. Bussolati G., Pearse A. G. Immunofluorescent localization of the gastrin-secreting G cells in the pyloric antrum of the pig. Histochemie. 1970;21(1):1–4. doi: 10.1007/BF00304798. [DOI] [PubMed] [Google Scholar]
  5. Graham R. C., Jr, Karnovsky M. J. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem. 1966 Apr;14(4):291–302. doi: 10.1177/14.4.291. [DOI] [PubMed] [Google Scholar]
  6. Greider M. H., Steinberg V., McGuigan J. E. Electron microscopic identification of the gastrin cell of the human antral mucosa by means of immunocytochemistry. Gastroenterology. 1972 Oct;63(4):572–583. [PubMed] [Google Scholar]
  7. Kendall P. A., Polak J. M., Pearse A. G. Carbodiimide fixation for immunohistochemistry: observations on the fixation of polypeptide hormones. Experientia. 1971 Sep 15;27(9):1104–1106. doi: 10.1007/BF02138909. [DOI] [PubMed] [Google Scholar]
  8. Mazurkiewicz J. E., Nakane P. K. Light and electron microscopic localization of antigens in tissues embedded in polyethylene glycol with a peroxidase labeled antibody method. J Histochem Cytochem. 1972 Dec;20(12):969–974. doi: 10.1177/20.12.969. [DOI] [PubMed] [Google Scholar]
  9. McGuigan J. E. Gastric mucosal intracellular localization of gastrin by immunofluorescence. Gastroenterology. 1968 Sep;55(3):315–327. [PubMed] [Google Scholar]
  10. McGuigan J. E., Greider M. H. Correlative immuncchemical and light microscopic studies of the gastrin cell of the antral mucosa. Gastroenterology. 1971 Feb;60(2):223–236. [PubMed] [Google Scholar]
  11. Moriarty G. C. Adenohypophysis: ultrastructural cytochemistry. A review. J Histochem Cytochem. 1973 Oct;21(10):855–894. doi: 10.1177/21.10.855. [DOI] [PubMed] [Google Scholar]
  12. Moriarty G. C., Moriarty C. M., Sternberger L. A. Ultrastructural immunocytochemistry with unlabeled antibodies and the peroxidase-antiperoxidase complex. A technique more sensitive than radioimmunoassay. J Histochem Cytochem. 1973 Sep;21(9):825–833. doi: 10.1177/21.9.825. [DOI] [PubMed] [Google Scholar]
  13. Pearse A. G., Bussolati G. Immunofluorescence studies on the distribution of gastrin cells in different clinical states. Gut. 1970 Aug;11(8):646–648. doi: 10.1136/gut.11.8.646. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Piris J., Whitehead R. An immunoperoxidase technique for the identification of gastrin-producing cells. J Clin Pathol. 1974 Oct;27(10):798–799. doi: 10.1136/jcp.27.10.798. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Polak J. M., Bloom S., Coulling I., Pearse A. G. Immunofluorescent localization of enteroglucagon cells in the gastrointestinal tract of the dog. Gut. 1971 Apr;12(4):311–318. doi: 10.1136/gut.12.4.311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Polak J. M., Bloom S., Coulling I., Pearse A. G. Immunofluorescent localization of secretin in the canine duodenum. Gut. 1971 Aug;12(8):605–610. doi: 10.1136/gut.12.8.605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Polak J. M., Doe W., Coulling I., Pearse A. G. The G cells in pernicious anaemia. Gut. 1971 Apr;12(4):319–323. doi: 10.1136/gut.12.4.319. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Polak J. M., Pearse A. G., Van Noorden S., Bloom S. R., Rossiter M. A. Secretin cells in coeliac disease. Gut. 1973 Nov;14(11):870–874. doi: 10.1136/gut.14.11.870. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Polak J. M., Stagg B., Pearse A. G. Two types of Zollinger-Ellison syndrome: immunofluorescent, cytochemical and ultrastructural studies of the antral and pancreatic gastrin cells in different clinical states. Gut. 1972 Jul;13(7):501–512. doi: 10.1136/gut.13.7.501. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES