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Equivalently overexpressed C43(DE3) cells were normalized to OD600nm = 1 and 5µl of the cell lysate was used for western blotting. Blot was developed with Penta-His HRP conjugate antibody (Qiagen). Lane1 – Marker, lane 2 – MgtA D373N BFP, Lane 3 – MgtA D373N GFP. The expression level of the BFP tagged construct is markedly lower than the GFP tagged construct.
