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. 1993 Oct 15;90(20):9485–9488. doi: 10.1073/pnas.90.20.9485

Measurement of the g-tensor of the P700+. signal from deuterated cyanobacterial photosystem I particles.

T F Prisner 1, A E McDermott 1, S Un 1, J R Norris 1, M C Thurnauer 1, R G Griffin 1
PMCID: PMC47593  PMID: 8415727

Abstract

We report high-field continuous wave EPR spectra of P700+. in preparations obtained from deuterated cyanobacteria (Synechococcus lividus). Measurements were performed with photosystem I (PS-I) preparations, whole cells from cyanobacteria grown in 2H2O, and photosystem II (PS-II) preparations, as well as with protonated PS-I preparations. Because of the significantly improved resolution of our 140-GHz spectrometer (as compared with X- or Q-band EPR) the principal values of the g-tensor of the primary donor P700+. could be resolved and measured with high accuracy as g11 = 2.00304, g22 = 2.00262, and g33 = 2.00232. Other signals arising from Mn2+ and a dark signal from PS-II at g approximately 2.00266 are distinguished from the P700+. g-tensor powder pattern. The measured g values are compared with those of several bacterial reaction center donors.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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