Abstract
Specimens from 495 patients attending Johannesburg hospitals and family planning clinics were examined for Trichomonas vaginalis by microscopy of Giemsa (GS), Papanicolaou (PAP), and acridine-orange (AO) stained smears, and by culture in Feinberg-Whittington medium. Culture, Pap and GS stained smears from vaginal swabs yielded fewer positives than AO stained smears. Although Pap-stained cytological smears gave the highest number of positives, in 30% of these cases the presence of T.vaginalis could not be confirmed by examination of vaginal swabs. Of the positive AO-stained smears, 93% were also positive by at least one other technique.
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- AMIES C. R., GARABEDIAN M. PREPARATION AND USE OF FIXATIVE SLIDES FOR THE DIAGNOSIS OF TRICHOMONAS VAGINALIS. J Clin Pathol. 1965 Jan;18:27–30. doi: 10.1136/jcp.18.1.27. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BICKIS I., VON BERTALANFFY L. Identification of cytoplasmic basophilia (ribonucleic acid) by fluorescence microscopy. J Histochem Cytochem. 1956 Sep;4(5):481–493. doi: 10.1177/4.5.481. [DOI] [PubMed] [Google Scholar]
- Cox P. J., Nicol C. S. Growth studies of various strains of T. vaginalis and possible improvements in the laboratory diagnosis of trichomoniasis. Br J Vener Dis. 1973 Dec;49(6):536–539. doi: 10.1136/sti.49.6.536. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DART L. H., Jr, TURNER T. R. Fluorescence microscopy in exfoliative cytology. Report of acridine orange examination of 5491 cases, with comparison by the Papanicolaou technic. Lab Invest. 1959 Nov-Dec;8:1513–1522. [PubMed] [Google Scholar]
- DUREL P., ROIRON V., SIBOULET A., BOREL L. J. Systemic treatment of human trichomoniasis with a derivative of nitro-imidazole, 8823 RP. Br J Vener Dis. 1960 Mar;36:21–26. doi: 10.1136/sti.36.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FEINBERG J. G., WHITTINGTON M. J. A culture medium for Trichomonas vaginalis donné and species of Candida. J Clin Pathol. 1957 Nov;10(4):327–329. doi: 10.1136/jcp.10.4.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FREEMAN F. A modified staining technique for Trichomonas vaginalis. S Afr Med J. 1958 Dec 27;32(52):1235–1235. [PubMed] [Google Scholar]
- Hess J. Review of current methods for the detection of Trichomonas in clinical material. J Clin Pathol. 1969 May;22(3):269–272. doi: 10.1136/jcp.22.3.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hughes H. E., Gordon A. M., Barr G. T. A clinical and laboratory study of trichomoniasis of the female genital tract. J Obstet Gynaecol Br Commonw. 1966 Oct;73(5):821–827. doi: 10.1111/j.1471-0528.1966.tb06091.x. [DOI] [PubMed] [Google Scholar]
- LOWE G. H. A COMPARISON OF CURRENT LABORATORY METHODS AND A NEW SEMI-SOLID CULTURE MEDIUM FOR THE DETECTION OF TRICHOMONAS VAGINALIS. J Clin Pathol. 1965 Jul;18:432–434. doi: 10.1136/jcp.18.4.432. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lowe G. H. A comparison of culture media for the isolation of Trichomonas vaginalis. Med Lab Technol. 1972 Oct;29(4):389–391. [PubMed] [Google Scholar]
- McCann J. S. Comparison of direct microscopy and culture in the diagnosis of trichomoniasis. Br J Vener Dis. 1974 Dec;50(6):450–452. doi: 10.1136/sti.50.6.450. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morton R. S. Metronidazole in the single-dose treatment of trichomoniasis in men and women. Br J Vener Dis. 1972 Dec;48(6):525–527. doi: 10.1136/sti.48.6.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Perl G. Errors in the diagnosis of trichomonas vaginalis infections as observed among 1199 patients. Obstet Gynecol. 1972 Jan;39(1):7–9. [PubMed] [Google Scholar]
- Rayner C. F. Comparison of culture media for the growth of Trichomonas vaginalis. Br J Vener Dis. 1968 Mar;44(1):63–66. doi: 10.1136/sti.44.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thin R. N., Atia W., Parker J. D., Nicol C. S., Canti G. Value of Papanicolaou-stained smears in the diagnosis of trichomoniasis, candidiasis, and cervical herpes simplex virus infection in women. Br J Vener Dis. 1975 Apr;51(2):116–118. doi: 10.1136/sti.51.2.116. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WHITTINGTON M. J. Epidemiology of infections with Trichomonas vaginalis in the light of improved diagnostic methods. Br J Vener Dis. 1957 Jun;33(2):80–91. doi: 10.1136/sti.33.2.80. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Woodcock K. R. Treatment of trichomonal vaginitis with a single oral dose of metronidazole. Br J Vener Dis. 1972 Feb;48(1):65–68. doi: 10.1136/sti.48.1.65. [DOI] [PMC free article] [PubMed] [Google Scholar]