Abstract
Serum agglutinins of chick and pigeon cells are predominantly immunoglobulin M and can be inactivated by 2 mercaptoethanol. 2 Mercaptoethanol is more effective than strong suspensions of red cells in removing the agglutinins of indicator cells from sera being prepared for HAI tests. In rubella HAI tests from a dilution of 1 to 10 dilute 2 mercaptoethanol offer a convenient method of removing agglutinins, and, at higher concentration, allow dilutions of sera from 1 in 2-5 to be tested without significant interference by heteroagglutinins. HAI titres are comparable when red cell absorbed and 2 mercaptoethanol treated sera are tested in parallel.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Biano S. A., Chang T. W., Daniels J. B. Rubella hemagglutination inhibition: removal of nonspecific agglutination due to manganous chloride. Appl Microbiol. 1974 Dec;28(6):992–994. doi: 10.1128/am.28.6.992-994.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hambling M. H. Effect of a vaccination programme on the distribution of rubella antibodies in women of childbearing age. Lancet. 1975 May 17;1(7916):1130–1133. doi: 10.1016/s0140-6736(75)92514-3. [DOI] [PubMed] [Google Scholar]
- Hatch F. T. Practical methods for plasma lipoprotein analysis. Adv Lipid Res. 1968;6:1–68. [PubMed] [Google Scholar]
- Haukenes G. A rubella haemagglutination inhibitor simulating antibody. Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Dec;81(6):719–723. doi: 10.1111/j.1699-0463.1973.tb02266.x. [DOI] [PubMed] [Google Scholar]
- Plotkin S. A., Farquhar J. D. Immunity to rubella: comparison between naturally and artificially induced resistance. Postgrad Med J. 1972 Jul;48(Suppl):47–54. [PubMed] [Google Scholar]
- Saeed A. A., Murray H. G. Letter: A non-specific inhibitor or rubella haemagglutinn in day-old chick cells. Lancet. 1975 Aug 30;2(7931):413–414. doi: 10.1016/s0140-6736(75)92932-3. [DOI] [PubMed] [Google Scholar]