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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: Virology. 2015 Dec 18;489:51–62. doi: 10.1016/j.virol.2015.12.001

Figure 4.

Figure 4

NCL recombinant protein binds to 5′ terminal region and 3′ UTR from the FCV genomic RNA. A) Schematic representation of NCL and ΔrNCL proteins. The RNA binding domains (RBD), nuclear localization signal (NLS), and the GAR domain are highlighted. N-glycosylation sites, located at positions 317–319, 399–401, 403–405, 477–479, and 491–493 are indicated. GST or GFP are located in the N-terminal end of the ΔrNCL protein. B) The [α-32P] UTP-labeled RNAs corresponding to the 5′ terminal region or C) the 3′ UTR of the FCV genomic RNA, were interacted with increasing amounts of ΔrNCL, (lanes 2, 3 and 4 respectively), or with the rGST protein (lanes 5). Lanes 1: free RNA. Migration of complexes is indicated.