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Components of functionalization solution for PA gels. All volumes are listed as microliter amounts.
| ddH2O | 0.5 M HEPES NaOH pH = 6.0 |
100% ethanol |
0.2% Bis- acrylamide |
0.2% tetramethacrylate in ethanol |
0.03% N6 in 50% ethanol |
3% Irgacure in ethanol |
Total volume |
|---|---|---|---|---|---|---|---|
| 433.3 | 100 | 65 | 50 | 10 | 333.3 | 8.33 | 1000 |
