Skip to main content
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1976 Nov;29(11):991–994. doi: 10.1136/jcp.29.11.991

Improved antibody detection by the use of range expansion and longer filter wavelength in a low ionic strength-protamine sulphate Auto-Analyzer system.

D M Downie, D Voak
PMCID: PMC476253  PMID: 1002844

Abstract

Range expansion, achieved by insertion of a variable resistance between the colorimeter and the recorder together with the use of 550 nm colorimeter filters, has resulted in markedly improved sensitivity for antibody detection, and improved sample identification, in a low ionic strength-protamine sulphate (LISPS) system. Range expansion also permits a lower concentration of red cells to be used, thus economizing on fully typed cells. Glycerol stored frozen cells were found to be only slightly less sensitive than fresh cells in this system.

Full text

PDF
991

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Annan W., Fisher G. W. A range expander unit for AutoAnalyzer recorders. Med Lab Technol. 1971 Jul;28(3):308–309. [PubMed] [Google Scholar]
  2. Casey F. M., Dodd B. E., Lincoln P. J. A study of the characteristics of certain Rh antibodies preferentially detactable by enzyme technique. Vox Sang. 1972 Dec;23(6):493–507. doi: 10.1111/j.1423-0410.1972.tb03843.x. [DOI] [PubMed] [Google Scholar]
  3. Dawes B. J., Goldsmith K. L. Automated red cell antibody detection. An improved method. Vox Sang. 1975;29(4):248–252. doi: 10.1111/j.1423-0410.1975.tb00505.x. [DOI] [PubMed] [Google Scholar]
  4. Greenwalt T. J., Steane E. A. Quantitative haemagglutination. 6. Relationship of sialic acid content of red cells and aggregation by Polybrene, protamine and poly-L-lysine. Br J Haematol. 1973 Aug;25(2):227–237. doi: 10.1111/j.1365-2141.1973.tb01734.x. [DOI] [PubMed] [Google Scholar]
  5. Gunson H. H., Phillips P. K., Stratton F. The effect of surfactant in the auto-analyser. Vox Sang. 1972;22(2):183–187. doi: 10.1111/j.1423-0410.1972.tb05124.x. [DOI] [PubMed] [Google Scholar]
  6. Habibi B., Gerbal A., Salmon C. A papain-bromelin-polybrene four-channel autoanalyzer system for blood group antibody screening. Analysis of 22,912 sera. Vox Sang. 1973 Oct;25(4):289–297. doi: 10.1111/j.1423-0410.1973.tb04376.x. [DOI] [PubMed] [Google Scholar]
  7. Kolberg J., Nordhagen R. An evaluation of some factors affecting the detection of blood group antibodies by automated methods. Transfusion. 1975 Jul-Aug;15(4):334–339. doi: 10.1046/j.1537-2995.1975.15476034553.x. [DOI] [PubMed] [Google Scholar]
  8. Lalezari P. A mew method for detection of red blood cell antibodies. Transfusion. 1968 Nov-Dec;8(6):372–380. doi: 10.1111/j.1537-2995.1968.tb02439.x. [DOI] [PubMed] [Google Scholar]
  9. Marsh W. L., Nichols M., Jenkins W. J. Automated detection of blood group antibodies. J Med Lab Technol. 1968 Oct;25(4):335–342. [PubMed] [Google Scholar]
  10. Moore B. P. Automation in the blood transfusion laboratory: I. Antibody dectection and quantitation in the technicon autoanalyzer. Can Med Assoc J. 1969 Feb 22;100(8):381–387. [PMC free article] [PubMed] [Google Scholar]
  11. Owen J. A. Range expansion in auto-analyser recorders. Clin Chim Acta. 1970 Jul;29(1):89–91. doi: 10.1016/0009-8981(70)90226-3. [DOI] [PubMed] [Google Scholar]
  12. Perrault R., Högman C. Automated red cell antibody analysis. A parallel study. I. Detection and quantitation. Vox Sang. 1971 Apr;20(4):340–355. doi: 10.1111/j.1423-0410.1971.tb00452.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES