Abstract
A spectrophotometric method using umbelliferone derivatives has been developed for the study of enzymic activites of mycobacteria. Arylsulphatase activity can be estimated in six hours rather than in 10 days as in the conventional method. The technique may be applied to a study of whole cells or to cell-free extracts.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Diaz G. A., Wayne L. G. Clarification of sonic lysates of Mycobacterium tuberculosis. Am Rev Respir Dis. 1974 Jan;109(1):154–155. doi: 10.1164/arrd.1974.109.1.154. [DOI] [PubMed] [Google Scholar]
- Maddocks J. L., Greenan M. J. A rapid method for identifying bacterial enzymes. J Clin Pathol. 1975 Aug;28(8):686–687. doi: 10.1136/jcp.28.8.686. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stanford J. L., Grange J. M. The meaning and structure of species as applied to mycobacteria. Tubercle. 1974 Jun;55(2):143–152. doi: 10.1016/0041-3879(74)90008-7. [DOI] [PubMed] [Google Scholar]
- Tacquet A., Tison F., Polspoel B., Roos P., Devulder B. Etude de la beta-D-galactosidase des mycobactéries. (Note préliminaire) Ann Inst Pasteur (Paris) 1966 Jul;111(1):86–89. [PubMed] [Google Scholar]
- WHITEHEAD J. E., WILDY P., ENGBAEK H. C. Arylsulphatase activity of mycobacteria. J Pathol Bacteriol. 1953 Apr;65(2):451–460. doi: 10.1002/path.1700650217. [DOI] [PubMed] [Google Scholar]