Abstract
Thirty-three strains of bacteria (30 Enterobacteriaceae and one strain each of Aeromonas formicans, A. hydrophila, and Plesiomonas shigelloides) were tested three times in each of 27 conventional tests and in the API, Enterotube, and Pathotec systems. The results obtained were analysed for test reproducibility within each kit, correlation of the kit tests with the equivalent conventional media, and the identification of the strains by the kits. Difficulties in evaluation and comparison of identifications are discussed. A practical evaluation of the kits was also made.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brooks K. A., Jens M., Sodeman T. M. A clinical evaluation of the API microtube system for identification of Enterobacteriaceae. Am J Med Technol. 1974 Feb;40(2):55–61. [PubMed] [Google Scholar]
- Butler D. A., Lobregat C. M., Gavan T. L. Reproducibility of the analytab (API 20E) system. J Clin Microbiol. 1975 Oct;2(4):322–326. doi: 10.1128/jcm.2.4.322-326.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COCHRAN W. G. The comparison of percentages in matched samples. Biometrika. 1950 Dec;37(3-4):256–266. [PubMed] [Google Scholar]
- Hayek L. J., Willis G. W. A comparison of two commerical methods for the identification of the Enterobacteriaceae--API 20E and the Enterotube--with conventional methods. J Clin Pathol. 1976 Feb;29(2):158–161. doi: 10.1136/jcp.29.2.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lapage S. P., Bascomb S., Willcox W. R., Curtis M. A. Identification of bacteria by computer: general aspects and perspectives. J Gen Microbiol. 1973 Aug;77(2):273–290. doi: 10.1099/00221287-77-2-273. [DOI] [PubMed] [Google Scholar]
- Nord C. E., Lindberg A. A., Dahlbäck A. Evaluation of five test-kits-API, AuxoTab, Enterotube, PathoTec and R-B-for identification of Enterobacteriaceae. Med Microbiol Immunol. 1974 Mar 22;159(3):211–220. doi: 10.1007/BF02121337. [DOI] [PubMed] [Google Scholar]
- Sneath P. H., Johnson R. The influence on numerical taxonomic similarities of errors in microbiological tests. J Gen Microbiol. 1972 Sep;72(2):377–392. doi: 10.1099/00221287-72-2-377. [DOI] [PubMed] [Google Scholar]
- Snell J. J., Lapage S. P. Carbon source utilization tests as an aid to the classification of non-fermenting gram-negative bacteria. J Gen Microbiol. 1973 Jan;74(1):9–20. doi: 10.1099/00221287-74-1-9. [DOI] [PubMed] [Google Scholar]