(
A) Overlay of the side views of the S3-S6 segments of one TRPV1 subunit and the S5-S6 segments of an adjacent subunit in the apo (subunit 1, S3-S4 in light pink, S5-S6 in magenta; subunit 2, S5-S6 in light grey) and the DkTx/RTx bound state (subunit 1, S3-S4 in light blue, S5-S6 in teal; subunit 2, S5-S6 in light orange). The structures shown are the refined structural models of TRPV1 from (
Bae et al., 2016), with the docked solution structure of DkTx (K1 in green and K2 in cyan). E600 is shown in stick representation and colored in dark blue, and the red highlight denotes the location from which the pore turret was deleted in the structure used for structure determination (
Cao et al., 2013;
Liao et al., 2013). (
B) Mean P
o-T relation (mean ± SEM, n = 9, data from
Figure 10,) for WT TRPV1 obtained in the presence of external Na
+ and DkTx. The P
o-T relations for individual cells are shown as colored continuous curves.