Skip to main content
NCBI home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1977 Jun;30(6):510–517. doi: 10.1136/jcp.30.6.510

Modification of the microimmunofluorescence test to provide a routine serodiagnostic test for chlamydial infection.

J D Treharne, S Darougar, B R Jones
PMCID: PMC476459  PMID: 326816

Abstract

A modification of the microimmunofluorescence test to provide a practicable routine serodiagnostic test for detecting and characterising chlamydial infection is described which uses four antigen pools, one of which corresponds with each of the four main clinical and epidemiological types of chlamydial infection. The three subgroup A Chlamydia (Chlamydia trachomatis) pools are: pool 1, hyperendemic trachoma TRIC agent serotypes A, B, and C; pool 2, paratrachoma TRIC agent serotypes D, E, F, G, H, I, and K; pool 3, lymphogranuloma venereum (LGV) agent serotypes L1, L2, and L3. Pool 4 contained four representative isolates of subgroup B Chlamydia (Chlamydia psittaci). For routine purposes sera need be screened only against these four representative antigen pools. This will detect antibody and indicate which clinical and epidemiological type of chlamydial infection is implicated, thereby clearly distinguishing those infections that are due to C. psittaci. The pattern of the cross-reactions may indicate the individual serotype involved, and further titration requiring a maximum of four individual antigens is sufficient to determine the serotype. The slight loss in sensitivity (twofold) is more than compensated for by the reduction in cost and the tenfold increase in the total number of sera which can be examined.

Full text

PDF
510

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Briones O. C., Hanna L., Jawetz E., Dawson C. R., Ostler H. B. Type-specific antibodies in human Chlamydia trachomatis infections of the eye. J Immunol. 1974 Oct;113(4):1262–1270. [PubMed] [Google Scholar]
  2. Dwyer R. S., Treharne J. D., Jones B. R., Herring J. Chlamydial infection. Results of micro-immunofluorescence tests for the detection of type-specific antibody in certain chlamydial infections. Br J Vener Dis. 1972 Dec;48(6):452–459. doi: 10.1136/sti.48.6.452. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hanna L., Jawetz E., Nabli B., Hoshiwara I., Ostler B., Dawson C. Titration and typing of serum antibodies in TRIC infections by immunofluorescence. J Immunol. 1972 Jan;108(1):102–107. [PubMed] [Google Scholar]
  4. Jones B. R. The prevention of blindness from trachoma. Trans Ophthalmol Soc U K. 1975 Apr;95(1):16–33. [PubMed] [Google Scholar]
  5. Jones B. R., Treharne J. D. Micro-immunofluorescence type-specific serological tests for chlamydial infection applied to psittacosis, ornithosis, lymphogranuloma venereum, trachoma, paratrachoma and 'nonspecific' urethritis. Proc R Soc Med. 1974 Aug;67(8):735–736. [PMC free article] [PubMed] [Google Scholar]
  6. MURRAY E. S. GUINEA PIG INCLUSION CONJUNCTIVITIS VIRUS. I. ISOLATION AND IDENTIFICATION AS A MEMBER OF THE PSITTACOSIS-LYMPHOGRANULOMA-TRACHOMA GROUP. J Infect Dis. 1964 Feb;114:1–12. doi: 10.1093/infdis/114.1.1. [DOI] [PubMed] [Google Scholar]
  7. Philip R. N., Casper E. A., Gordon F. B., Quan A. L. Fluorescent antibody responses to chlamydial infection in patients with lymphogranuloma venereum and urethritis. J Immunol. 1974 Jun;112(6):2126–2134. [PubMed] [Google Scholar]
  8. Schachter J., Meyer K. F. Lymphogranuloma venereum. II. Characterization of some recently isolated strains. J Bacteriol. 1969 Sep;99(3):636–638. doi: 10.1128/jb.99.3.636-638.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Treharne J. D., Darougar S., Jones B. R. Characterization of a further microimmunofluorescence serotype of Chlamydia: TRIC type G. Br J Vener Dis. 1973 Jun;49(3):295–300. doi: 10.1136/sti.49.3.295. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Wang S. P., Grayston J. T., Gale J. L. Three new immunologic types of trachoma-inclusion conjunctivitis organisms. J Immunol. 1973 Mar;110(3):873–879. [PubMed] [Google Scholar]
  11. Wang S. P., Grayston J. T. Human serology in Chlamydia trachomatis infection with microimmunofluorescence. J Infect Dis. 1974 Oct;130(4):388–397. doi: 10.1093/infdis/130.4.388. [DOI] [PubMed] [Google Scholar]
  12. Wang S. P., Grayston J. T. Immunologic relationship between genital TRIC, lymphogranuloma venereum, and related organisms in a new microtiter indirect immunofluorescence test. Am J Ophthalmol. 1970 Sep;70(3):367–374. doi: 10.1016/0002-9394(70)90096-6. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES