Fig. 4.
The synaptic GABAARs of P20 – 24 L2/3 cortical pyramidal neurons are neurosteroid sensitive and furthermore, P20 – 24 cortical brain slices can synthesise GABAAR-active neurosteroids. A). Averaged, superimposed mIPSCs normalised with respect to peak amplitude, recorded from representative WT L2/3 cortical pyramidal neurons from brain slices derived from P20 - 24 mice, under control conditions and following incubation (>120 min) with 100 nM 5α3α. B) Averaged, superimposed P20-24 mIPSCs normalised with respect to peak amplitude, recorded under control conditions and following incubation (>120 min) with 3 μM 5α-DHP; co-incubation with 100 μM indomethacin (Indo) and 3 μM 5α-DHP (>120 min); or incubation with 3 μM 5α-DHP (>120 min) followed by 0.5 mM ICS γ-CD. C) Summary bar graph illustrating the increase in the mIPSC τw in response to incubation of 100 nM 5α3α; 5α-DHP incubation and the much reduced 5α-DHP effect following either co-incubation with 100 μM indomethacin (Indo), or addition of 0.5 mM γ-CD to the ICS (n = 5–25 cells). *** = p < 0.001 vs control, one way ANOVA with Tukey post hoc analysis.