Abstract
The recovery of anaerobic, facultative anaerobic, and aerobic pathogens from very small inocula was studied under conditions that could be related to routine blood culture procedures. Results with Brain Heart Infusion broth were unsatisfactory. Freshly prepared Brewer thioglycollate medium gave apparently good results, but there are disadvantages when this medium is used for blood culture. Results with Difco Thiol broth were disappointing. A modification of Robertson's cooked-meat broth supplemented with Brain Heart Infusion gave good recovery and sustained viability with a wide range of test organisms including exacting strains. The findings raise points of practical importance.
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- Blazevic D. J., Stemper J. E., Matsen J. M. Effect of aerobic and anaerobic atmospheres on isolation of organisms from blood cultures. J Clin Microbiol. 1975 Feb;1(2):154–156. doi: 10.1128/jcm.1.2.154-156.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brooks K., Sodeman T. Rapid detection of bacteremia by a radiometric system. A clinical evaluation. Am J Clin Pathol. 1974 Jun;61(6):859–866. doi: 10.1093/ajcp/61.6.859. [DOI] [PubMed] [Google Scholar]
- Chow A. W., Guze L. B. Bacteroidaceae bacteremia: clinical experience with 112 patients. Medicine (Baltimore) 1974 Mar;53(2):93–126. [PubMed] [Google Scholar]
- Collee J. G., Watt B., Fowler E. B., Brown R. An evaluation of the Gaspak system in the culture of anaerobic bacteria. J Appl Bacteriol. 1972 Mar;35(1):71–82. doi: 10.1111/j.1365-2672.1972.tb03675.x. [DOI] [PubMed] [Google Scholar]
- Duerden B. I., Holbrook W. P., Collee J. G., Watt B. The characterization of clinically important gram negative anaerobic bacilli by conventional bacteriological tests. J Appl Bacteriol. 1976 Apr;40(2):163–188. doi: 10.1111/j.1365-2672.1976.tb04164.x. [DOI] [PubMed] [Google Scholar]
- Forgan-Smith W. R., Darrell J. H. A comparison of media used in vitro to isolate non-sporing Gram-negative anaerobes from blood. J Clin Pathol. 1974 Apr;27(4):280–283. doi: 10.1136/jcp.27.4.280. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gantz N. M., Medeiros A. A., Swain J. L., O'Brien T. F. Vacuum blood-culture bottles inhibiting growth of Candida and fostering growth of Bacteroides. Lancet. 1974 Nov 16;2(7890):1174–1176. doi: 10.1016/s0140-6736(74)90813-7. [DOI] [PubMed] [Google Scholar]
- Shanson D. C. An experimental assessment of different anaerobic blood culture methods. J Clin Pathol. 1974 Apr;27(4):273–279. doi: 10.1136/jcp.27.4.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Shanson D. C., Barnicoat An experimental comparison of Thiol broth with Brewer's thioglycollate for anaerobic blood cultures. J Clin Pathol. 1975 May;28(5):407–409. doi: 10.1136/jcp.28.5.407. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith A. G., Little R. R. Detection of bacteremia by an automated radiometric method and a tubed broth method. Ann Clin Lab Sci. 1974 Nov-Dec;4(6):448–455. [PubMed] [Google Scholar]
- Szawatkowski M. V. A comparison of three readily available types of anaerobic blood culture media. Med Lab Sci. 1976 Jan;33(1):5–12. [PubMed] [Google Scholar]
- Thiemke W. A., Wicher K. Laboratory experience with a radiometric method for detecting bacteremia. J Clin Microbiol. 1975 Mar;1(3):302–308. doi: 10.1128/jcm.1.3.302-308.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Washington J. A., 2nd Comparison of two commercially available media for detection of bacteremia. Appl Microbiol. 1971 Oct;22(4):604–607. doi: 10.1128/am.22.4.604-607.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watt B., Hoare M. V., Collee J. G. Some variables affecting the recovery of anaerobic bacteria: a quantitative study. J Gen Microbiol. 1973 Aug;77(2):447–454. doi: 10.1099/00221287-77-2-447. [DOI] [PubMed] [Google Scholar]
- Wood N. G. Comparative study of two systems for detecting becteraemia and septicaemia. J Clin Pathol. 1976 Jun;29(6):530–533. doi: 10.1136/jcp.29.6.530. [DOI] [PMC free article] [PubMed] [Google Scholar]