Abstract
Hepatitis B core antigen (HBcAg) was prepared from human liver tissue and used in an immunoelectro-osmophoresis screening test to detect antibody to HBcAg (anti-HBc) in patients with evidence of liver disease and in blood donors. With the exception of two immunosuppressed HBsAg carriers, anti-HBc was found in all cases of hepatitis B infection even when HBsAg was detectable only by radioimmunoassay. Non-specific reactions were observed in 'non-B' hepatitis but, in spite of this problem, antib-HBc screening was considered a useful addition to routine tests in the clinical hepatitis laboratory.
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- Almeida J. D., Gay F. W., Wreghitt T. G. Pitfalls in the study of hepatitis A. Lancet. 1974 Sep 28;2(7883):748–751. doi: 10.1016/s0140-6736(74)90943-x. [DOI] [PubMed] [Google Scholar]
- Brzosko W. J., Madaliński K., Krawczyński K., Nowoslawski A. Duality of hepatitis B antigen and its antibody. I. Immunofluorescence studies. J Infect Dis. 1973 Apr;127(4):424–428. doi: 10.1093/infdis/127.4.424. [DOI] [PubMed] [Google Scholar]
- Budkowska A., Walicka B., Domaniewska G., Brzosko W. J. Letter: Immunoelectro-osmophoresis for detection of antibody to hepatitis B core antigen. N Engl J Med. 1974 Jun 27;290(26):1489–1490. doi: 10.1056/NEJM197406272902618. [DOI] [PubMed] [Google Scholar]
- Hoofnagle J. H., Gerety R. J., Barker L. F. Antibody to hepatitis-B-virus core in man. Lancet. 1973 Oct 20;2(7834):869–873. doi: 10.1016/s0140-6736(73)92004-7. [DOI] [PubMed] [Google Scholar]
- Magnius L. O., Espmark J. A. New specificities in Australia antigen positive sera distinct from the Le Bouvier determinants. J Immunol. 1972 Nov;109(5):1017–1021. [PubMed] [Google Scholar]
- Markenson J. A., Gerety R. J., Hoofnagle J. H., Barker L. F. Effects of cyclophosphamide on hepatitis B virus infection and challenge in chimpanzees. J Infect Dis. 1975 Feb;131(2):79–87. doi: 10.1093/infdis/131.2.79. [DOI] [PubMed] [Google Scholar]
- Moritsugu Y., Gold J. W., Wagner J., Dodd R. Y., Purcell R. H. Hepatitis B core antigen. Detection of antibody by radioimmunoprecipitation. J Immunol. 1975 Jun;114(6):1792–1798. [PubMed] [Google Scholar]