Abstract
A procedure is described for performing a functional assay of serum antithrombin activity. The method consists of adding serum to a thrombin solution and measuring, after a fixed incubation time, the residual thrombin activity on a substrate plasma. The mean serum antithrombin activity found in 96 healthy blood donors using this procedure was 109.5% (range 82%-160%). The method was linear over an activity range between 30.5% and 176%, was significantly correlated with antithrombin-III protein concentration determined by radial immunodiffusion (r = 0.86, P less than 0.01), and showed good reproducibility (coefficient of variation 2.7%). On account of its simplicity and precision this functional assay should be of considerable use in evaluating hypercoagulability.
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Selected References
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- Abildgaard U., Fagerhol M. K., Egeberg O. Comparison of progressive antithrombin activity and the concentration of three thrombin inhibitors in human plasma. Scand J Clin Lab Invest. 1970 Dec;26(4):349–354. doi: 10.3109/00365517009046245. [DOI] [PubMed] [Google Scholar]
- Bick R. L., Kovacs I., Fekete L. F. A new two-stage functional assay for antithrombin-III (heparin cofactor): clinical and laboratory evaluation. Thromb Res. 1976 Jun;8(6):745–756. doi: 10.1016/0049-3848(76)90003-7. [DOI] [PubMed] [Google Scholar]
- EGEBERG O. INHERITED ANTITHROMBIN DEFICIENCY CAUSING THROMBOPHILIA. Thromb Diath Haemorrh. 1965 Jun 15;13:516–530. [PubMed] [Google Scholar]
- Innerfield I., Goldfischer J. D., Reicher-Reiss H., Greenberg J. Serum antithrombin in coronary-artery disease. Am J Clin Pathol. 1976 Jan;65(1):64–68. doi: 10.1093/ajcp/65.1.64. [DOI] [PubMed] [Google Scholar]
- Odegård O. R., Teien A. N. Antithrombin III, heparin cofactor and antifactor Xa in a clinical material. Thromb Res. 1976 Feb;8(2):173–178. doi: 10.1016/0049-3848(76)90260-7. [DOI] [PubMed] [Google Scholar]
- Von Kaulla E., Von Kaulla K. N. Antithrombin 3 and diseases. Am J Clin Pathol. 1967 Jul;48(1):69–80. doi: 10.1093/ajcp/48.1.69. [DOI] [PubMed] [Google Scholar]
- Yin E. T., Wessler S., Stoll P. J. Identity of plasma-activated factor X inhibitor with antithrombin 3 and heparin cofactor. J Biol Chem. 1971 Jun 10;246(11):3712–3719. [PubMed] [Google Scholar]