Abstract
A recently available kit for the determination of rubella haemagglutination inhibiting antibodies (Rubindex, Ortho diagnostics) was was compared with the method used routinely in this laboratory. Rubindex compared favourably in that no significant difference in titre was found in 95.1% of 287 sera tested. Difficulty occurred with some sera from cases of Mycoplasma pneumoniae infection, rheumatoid arthritis, and a small number of normal sera containing human "O" cell agglutinins. This was overcome by treatment of the sera with 2-mercaptoethanol. Rubindex had the advantage that all reagents were provided in kit form and the trypsinised human "O" cells provided had a longer shelf life than chick erythrocytes.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bannerman C. M., Ross C. A. Letter: Trypsin-treated human erythrocytes for rubella hemagglutination inhibition tests. J Clin Pathol. 1973 Oct;26(10):807–808. doi: 10.1136/jcp.26.10.807-b. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Biddle F. The unmasking of receptors for rubella virus by trypsinization of red cells. Microbios. 1971 Apr;3(12):255–260. [PubMed] [Google Scholar]
- Caul E. O., Smyth G. W., Clarke S. K. A simplified method for the detection of rubella-specific IgM employing sucrose density fractionation and 2-mercaptoethanol. J Hyg (Lond) 1974 Dec;73(3):329–340. doi: 10.1017/s0022172400042674. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Halonen P. E., Ryan J. M., Stewart J. A. Rubella hemagglutinin prepared with alkaline extraction of virus grown in suspension culture of BHK-21 cells. Proc Soc Exp Biol Med. 1967 May;125(1):162–167. doi: 10.3181/00379727-125-32038. [DOI] [PubMed] [Google Scholar]
- Holms I. H., Warburton M. F. Is rubella an arbovirus? Lancet. 1967 Dec 9;2(7528):1233–1236. doi: 10.1016/s0140-6736(67)90568-5. [DOI] [PubMed] [Google Scholar]
- Iwakata S., Morrissey L. P., Rhodes A. J., Labzoffsky N. A. The use of trypsin-treated human group O erythrocytes in rubella hemagglutination-inhibition test. Arch Gesamte Virusforsch. 1974;45(4):352–361. doi: 10.1007/BF01242878. [DOI] [PubMed] [Google Scholar]
- Mortimer P. P. An alternative method for inactivating heteroagglutinins in human sera applicable to rubella haemagglutination inhibition testing at low dilutions. J Clin Pathol. 1976 May;29(5):417–422. doi: 10.1136/jcp.29.5.417. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nelson D. B., Quirin P., Inhorn S. L. Compatibility of trypsin-modified human erythrocytes in the rubella hemagglutination-inhibition test employing three serum treatment procedures. Appl Microbiol. 1974 Apr;27(4):767–770. doi: 10.1128/am.27.4.767-770.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Quirin E. P., Nelson D. B., Inhorn S. L. Use of trypsin-modified human erythrocytes in rubella hemagglutination-inhibition testing. Appl Microbiol. 1972 Sep;24(3):353–357. doi: 10.1128/am.24.3.353-357.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Saeed A. A., Murray H. G. Letter: A non-specific inhibitor or rubella haemagglutinn in day-old chick cells. Lancet. 1975 Aug 30;2(7931):413–414. doi: 10.1016/s0140-6736(75)92932-3. [DOI] [PubMed] [Google Scholar]
- Schmidt N. J., Lennette E. H. Variables of the rubella hemagglutination-inhibition test system and their effect on antigen and antibody titers. Appl Microbiol. 1970 Mar;19(3):491–504. doi: 10.1128/am.19.3.491-504.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Smith J. A., Cummins A. C. Evaluation of a rubella hemagglutination inhibition test system. J Clin Microbiol. 1976 Jan;3(1):5–7. doi: 10.1128/jcm.3.1.5-7.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stewart G. L., Parkman P. D., Hopps H. E., Douglas R. D., Hamilton J. P., Meyer H. M., Jr Rubella-virus hemagglutination-inhibition test. N Engl J Med. 1967 Mar 9;276(10):554–557. doi: 10.1056/NEJM196703092761006. [DOI] [PubMed] [Google Scholar]