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. 1977 Jan;30(1):29–34. doi: 10.1136/jcp.30.1.29

Avian erythrocyte agglutination tests with the sera of bird fanciers.

J A Diment, J Pepys
PMCID: PMC476629  PMID: 838868

Abstract

The agglutination of avian red cells, particularly pigeon and budgerigar cells, by sera from patients who had been exposed to birds and had respiratory symptoms, as well as from healthy bird fanciers, has been used to detect the presence of avian specific antibody. Agglutination tests performed by standard techniques using bird erythrocytes can be read within 45 minutes. One hundred and sixty-five sera were tested, and the 44 which were precipitin positive gave a positive agglutination test with one exception. Of 61 sera which gave positive agglutination tests, 43 gave positive precipitin tests with unconcentrated serum and seven others after fourfold concentration of the serum. Avian antibodies were demonstrated in the remaining 11 sera by radioimmunoelectrophoretic tests. Absorption of the sera with pigeon IgG and IgM and the use of animal antisera directed against pigeon IgG showed that the agglutination reactions were mediated by antibodies directed against pigeon IgG and IgM, and against light chains derived from pigeon IgG.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARBORIAK J. J., SOSMAN A. J., REED C. E. SEROLOGICAL STUDIES IN PIGEON BREEDER'S DISEASE. J Lab Clin Med. 1965 Apr;65:600–604. [PubMed] [Google Scholar]
  2. Bach C., Fournier C., Drouhet E., Texier J. L., Dardenne M., Laborde M. A., Bach J. F. La maladie des éleveurs d'oiseaux chez l'enfant. Mise en évidence d'une sensibilisation des lymphocytes sans hypersensibilité retardée. Presse Med. 1971 Feb 20;79(9):383–386. [PubMed] [Google Scholar]
  3. Berrens L., Maesen F. P. An immunochemical study of pigeon-breeder's disease. I. The aselective antigens. Int Arch Allergy Appl Immunol. 1972;43(2):289–304. doi: 10.1159/000230843. [DOI] [PubMed] [Google Scholar]
  4. CSIZMAS L. Preparation of formalinized erythrocytes. Proc Soc Exp Biol Med. 1960 Jan;103:157–160. doi: 10.3181/00379727-103-25444. [DOI] [PubMed] [Google Scholar]
  5. Cayzer I., Dane D. S., Cameron C. H., Denning J. V. A rapid haemagglutination test for hepatitis-B antigen. Lancet. 1974 May 18;1(7864):947–949. doi: 10.1016/s0140-6736(74)91259-8. [DOI] [PubMed] [Google Scholar]
  6. Edwards J. H., Barboriak J. J., Fink J. N. Antigens in pigeon breeders' disease. Immunology. 1970 Nov;19(5):729–734. [PMC free article] [PubMed] [Google Scholar]
  7. FLEISCHMAN J. B., PAIN R. H., PORTER R. R. Reduction of gamma-globulins. Arch Biochem Biophys. 1962 Sep;Suppl 1:174–180. [PubMed] [Google Scholar]
  8. Faux J. A., Wells I. D., Pepys J. Specificity of avian serum proteins in tests against the sera of bird fanciers. Clin Allergy. 1971 Jun;1(2):159–170. doi: 10.1111/j.1365-2222.1971.tb03015.x. [DOI] [PubMed] [Google Scholar]
  9. Faux J. A., Wide L., Hargreave F. E., Longbottom J. L., Pepys J. Immunological aspects of respiratory allergy in budgerigar (Melopsittacus undulatus) fanciers. Clin Allergy. 1971 Jun;1(2):149–158. doi: 10.1111/j.1365-2222.1971.tb03014.x. [DOI] [PubMed] [Google Scholar]
  10. Hargreave F. E., Pepys J., Longbottom J. L., Wraith D. G. Bird breeder's (fancier's) lung. Lancet. 1966 Feb 26;1(7435):445–449. doi: 10.1016/s0140-6736(66)91454-1. [DOI] [PubMed] [Google Scholar]
  11. Hunter W. M. Radioimmunoassay and saturation analysis. Preparation and assessment of radioactive tracers. Br Med Bull. 1974 Jan;30(1):18–23. doi: 10.1093/oxfordjournals.bmb.a071161. [DOI] [PubMed] [Google Scholar]
  12. Nielsen K. H., Parratt D., Boyd G., White R. G. Use of radiolabelled antiglobulin for quantitation of antibody to soluble antigens rendered particulate: application to human sera from 'pigeon fancier's lung syndrome'. Int Arch Allergy Appl Immunol. 1974;47(3):339–350. doi: 10.1159/000231227. [DOI] [PubMed] [Google Scholar]
  13. REED C. E., BARBEE R. A. PIGEON-BREEDERS' LUNG: A NEWLY OBSERVED INTERSTITIAL PULMONARY DISEASE. JAMA. 1965 Jul 26;193:261–265. doi: 10.1001/jama.1965.03090040005001. [DOI] [PubMed] [Google Scholar]
  14. Radermecker M., Bruwier M., François C., Brocteur J., Salmon J., André A., Van Caudwenberge H. Anti-P1 activity in pigeon breeders' serum . Clin Exp Immunol. 1975 Dec;22(3):546–549. [PMC free article] [PubMed] [Google Scholar]
  15. Sequeira P. J., Eldridge A. E. Treponemal haemagglutination test. Br J Vener Dis. 1973 Jun;49(3):242–248. doi: 10.1136/sti.49.3.242. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Soda K., Matumoto M., Tabata E. Erythrocytes of Japanese quail for hemagglutination by rubella virus. Jpn J Microbiol. 1971 Jul;15(4):351–357. doi: 10.1111/j.1348-0421.1971.tb00591.x. [DOI] [PubMed] [Google Scholar]
  17. Steinbuch M., Audran R. The isolation of IgG from mammalian sera with the aid of caprylic acid. Arch Biochem Biophys. 1969 Nov;134(2):279–284. doi: 10.1016/0003-9861(69)90285-9. [DOI] [PubMed] [Google Scholar]
  18. Travis J., Pannell R. Selective removal of albumin from plasma by affinity chromatography. Clin Chim Acta. 1973 Nov 23;49(1):49–52. doi: 10.1016/0009-8981(73)90341-0. [DOI] [PubMed] [Google Scholar]
  19. Weeke B. A manual of quantitative immunoelectrophoresis. Methods and applications. 1. General remarks on principles, equipment, reagents and procedures. Scand J Immunol Suppl. 1973;1:15–35. doi: 10.1111/j.1365-3083.1973.tb03776.x. [DOI] [PubMed] [Google Scholar]

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