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. 1977 Dec;30(12):1154–1157. doi: 10.1136/jcp.30.12.1154

An enzyme inhibition assay for 2,4-diamino-5-(3'4'-dichlorophenyl)-6-methylpyrimidine (DDMP,NSC 19494).

A H Calvert, J S Cridland, K R Harrap
PMCID: PMC476700  PMID: 604362

Abstract

An enzyme inhibiton method for the determination of serum levels of DDMP is described. This has proved to be a simple, practical, and reliable method for the clinical monitoring of patients.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERTINO J. R., FISCHER G. A. TECHNIQUES FOR STUDY OF RESISTANCE TO FOLIC ACID ANTAGONISTS. Methods Med Res. 1964;10:297–307. [PubMed] [Google Scholar]
  2. Cridland J. S., Weatherley B. C. Determination of 2,4-diamino-5-(3,4-dichlorophenyl)-6-methylpyrimidine (BW 197U) in human plasma. J Chromatogr. 1977 Jul 21;137(2):449–453. doi: 10.1016/s0021-9673(00)81367-9. [DOI] [PubMed] [Google Scholar]
  3. DIXON M. The determination of enzyme inhibitor constants. Biochem J. 1953 Aug;55(1):170–171. doi: 10.1042/bj0550170. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hill B. T., Goldie J. H., Price L. A. Studies concerned with overcoming resistance to methotrexate: a comparison of the effects of methotrexate and 2,4-diamino-5-(3',4'-dichlorophenyl)-6-methylpyrimidine (BW50197) on the colony forming ability of L5178Y cells. Br J Cancer. 1973 Sep;28(3):263–268. doi: 10.1038/bjc.1973.146. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Jackson R. C., Hart L. I., Harrap K. R. Intrinsic resistance to methotrexate of cultured mammalian cells in relation to the inhibition kinetics of their dihydrololate reductases. Cancer Res. 1976 Jun;36(6):1991–1997. [PubMed] [Google Scholar]
  6. MURPHY M. L., ELLISON R. R., KARNOFSKY D. A., BURCHENAL J. H. Clinical effects of the dichloro and monochlorophenyl analogues of diamino pyrimidine: antagonists of folic acid. J Clin Invest. 1954 Oct;33(10):1388–1396. doi: 10.1172/JCI103016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. McCormack J. J., Jaffe J. J. Dihydrofolate reductase from Trypanosoma equiperdum. II. Inhibition by 2,4-diaminopyrimidines and related heterocycles. J Med Chem. 1969 Jul;12(4):662–668. doi: 10.1021/jm00304a023. [DOI] [PubMed] [Google Scholar]
  8. Price L. A., Goldie J. H., Hill B. T. Methodichlorophen as anti-tumor drug. Br Med J. 1975 Apr 5;2(5961):20–21. doi: 10.1136/bmj.2.5961.20. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Simmons W. S., DeAngelis R. L. Quantitation of pyrimethamine and related diaminopyrimidines in situ by enhancement of fluorescence after thin layer chromatography. Anal Chem. 1973 Jul;45(8):1538–1540. doi: 10.1021/ac60330a056. [DOI] [PubMed] [Google Scholar]

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