Abstract
The results obtained by radial haemolysis in the detection of antibodies to rubella virus compared well with those obtained by haemagglutination inhibition. Radial haemolysis is unaffected by non-specific inhibitors. The sera do not therefore require pretreatment and the results are less equivocal. Radial haemolysis appears to be as sensitive as immunofluorescence and floatation centrifugation. It is possible to examine large numbers of sera with a considerable saving of time compared with the traditional haemagglutination technique. Rheumatoid factor may cause interference in radial haemolysis. Immune sera may be recorded as non-immune. This interference can be removed by 2-mercaptoethanol or reduced by heating sera at 60 degrees C for 20 minutes.
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Selected References
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- Grillner L., Strannegård O. Evaluation of the hemolysis-in-gel test for the screening of rubella immunity and the demonstration of recent infection. J Clin Microbiol. 1976 Feb;3(2):86–90. doi: 10.1128/jcm.3.2.86-90.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Quirin E. P., Nelson D. B., Inhorn S. L. Use of trypsin-modified human erythrocytes in rubella hemagglutination-inhibition testing. Appl Microbiol. 1972 Sep;24(3):353–357. doi: 10.1128/am.24.3.353-357.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schild G. C., Pereira M. S., Chakraverty P. Single-radial-hemolysis: a new method for the assay of antibody to influenza haemagglutinin. Applications for diagnosis and seroepidemiologic surveillance of influenza. Bull World Health Organ. 1975;52(1):43–50. [PMC free article] [PubMed] [Google Scholar]
- Skaug K., Orstavik I., Ulstrup J. C. Application of the passive haemolysis test for the determination of rubella virus antibodies. Acta Pathol Microbiol Scand B. 1975 Aug;83(4):367–372. doi: 10.1111/j.1699-0463.1975.tb00114.x. [DOI] [PubMed] [Google Scholar]
- Stewart G. L., Parkman P. D., Hopps H. E., Douglas R. D., Hamilton J. P., Meyer H. M., Jr Rubella-virus hemagglutination-inhibition test. N Engl J Med. 1967 Mar 9;276(10):554–557. doi: 10.1056/NEJM196703092761006. [DOI] [PubMed] [Google Scholar]
- Strannegård O., Grillner L., Lindberg I. M. Hemolysis-in-gel test for the demonstration of antibodies to rubella virus. J Clin Microbiol. 1975 Jun;1(6):491–494. doi: 10.1128/jcm.1.6.491-494.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]