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. 2015 Oct 19;6(40):42632–42650. doi: 10.18632/oncotarget.5822

Figure 4. Validation of ACSL4 effects on the mTOR pathway in MCF-7 cells.

Figure 4

Whole cell extracts were obtained as described for RPPA from MCF-7 Tet-Off empty vector, MCF-7 Tet-Off/ACSL4 and doxycycline-treated MCF-7 Tet-Off/ACSL4 cells (Doxy, 1 ug/ml, 48 h). Western blot was performed using the indicated antibodies. Representative blots A. and integrated optical density of protein levels B. quantified and normalized with the corresponding β-tubulin signal. As shown in Figure 1, data represent the means of fold changes ± SD of three independent experiments. a, b, c, d and e: ***p < 0.001; f and g: *p < 0.05 vs. MCF-7 Tet-Off empty vector cells.