Abstract
This paper describes the preparation of virus-infected cells for the detection of serum antibodies by the use of immunofluorescence techniques. The variations in virus growth in cells in relation to the length of incubation of cell monolayers and polio virus using two different fluorescein isothiocynate conjugates are discussed. It is shown that there is a critical time after virus infection when a definite positive immunofluorescent reaction is given. Furthermore, a method is described enabling the clinical pathologist to prepare infected cells with the use of an automatic tube inoculator at any time during 24 hours.
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Selected References
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- DARNELL J. E., Jr, LEVINTOW L., THOREN M. M., HOOPER J. L. The time course of synthesis of poliovirus RNA. Virology. 1961 Mar;13:271–279. doi: 10.1016/0042-6822(61)90145-3. [DOI] [PubMed] [Google Scholar]
- MAYOR H. D. Cytochemical and fluorescent antibody studies on the growth of poliovirus in tissue culture. Tex Rep Biol Med. 1961;19:106–122. [PubMed] [Google Scholar]
- Sander G. Storage of virus-infected tissue culture substrates for the detection of serum antibodies by immunofluorescence. J Clin Pathol. 1969 Nov;22(6):737–737. doi: 10.1136/jcp.22.6.737. [DOI] [PMC free article] [PubMed] [Google Scholar]