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. 2016 Feb 26;6:22193. doi: 10.1038/srep22193

Resistin Induces Hypertension and Insulin Resistance in Mice via a TLR4-Dependent Pathway

Yun Jiang 1, Linfang Lu 1, Youtao Hu 3, Qiang Li 2, Chaoqiang An 1, Xiaolan Yu 1, Le Shu 1, Ao Chen 1, Congcong Niu 1, Lei Zhou 2,a, Zaiqing Yang 1,b
PMCID: PMC4768137  PMID: 26917360

Abstract

Resistin, an adipokine involved in insulin resistance (IR) and diabetes, has recently been reported to play a role in cardiovascular events. However, its effect on blood pressure (BP) and the underlying mechanisms remain unclear. In the present study, we showed that resistin induces hypertension and IR in wild type (WT) mice, but not in tlr4−/− mice. Resistin upregulated angiotensinogen (Agt) expression in WT mice, whereas it had no effect on tlr4−/− mice, or in mice treated with the angiotensin-converting enzyme inhibitor perindopril. Real-time PCR and chromatin immunoprecipitation further confirmed that resistin activates the renin-angiotensin system (RAS) via the TLR4/P65/Agt pathway. This finding suggested an essential role of resistin in linking IR and hypertension, which may offer a novel target in clinic on the study of the association between diabetes and hypertension.

Hypertension, one of the independent risk factors for cardiovascular disease (CVD), affects approximately 70% of patients with diabetes, and the risk of CVD in diabetic patients is three times higher than that in healthy individuals1,2. Clinical studies suggest that insulin resistance (IR) and hyperinsulinemia, which often occur in patients with type 2 diabetes, are responsible for diabetes-associated hypertension3,4.

Resistin was first identified as an adipokine with a critical role in IR5,6,7. High plasma resistin levels have been reported in patients with CVD, indicating that increased resistin may be associated with both diabetes and CVD8. Accumulating evidence has provided insight into the function of resistin, and has implicated resistin in atherosclerosis, insulin-evoked vasodilation, and endothelial dysfunction, which are complications typically associated with hypertension8,9,10. These data suggest that resistin could be involved in the regulation of blood pressure (BP).

Toll-like receptor 4 (TLR4) is a putative resistin receptor that has been proposed to contribute to resistin-induced inflammation and IR11,12. However, whether resistin and TLR4 play a role in hypertension is largely unknown. In the present study, we investigated the effect of resistin on BP and IR in mice and elucidated the underlying mechanisms.

Results

Resistin induced hypertension and insulin resistance in wild-type mice

To examine the effect of resistin on hypertension, BP was measured in WT mice treated with resistin (Retn) or PBS (Con) using the tail-cuff method. Plasma resistin levels were higher in resistin-treated group than control group, indicating our resistin treatment is successful (Fig. 1A). Both systolic BP (SBP) and diastolic BP (DBP) were markedly higher in WT mice treated with resistin for 6 days [Fig. 1B, SBP: 104 ± 6 (Retn, D0), 140 ± 20 (Retn, D6) mmHg; DBP: 67 ± 12 (Retn, D0), 87 ± 15 (Retn, D6) mmHg], whereas no differences were observed in WT mice treated with PBS [Fig. 1B, SBP: 103 ± 6 (Con, D0), 101 ± 13 (Con, D6) mmHg; DBP: 69 ± 11 (Con, D0), 66 ± 8 (Con, D6) mmHg], indicating that resistin caused hypertension in WT mice. Resistin treatment increased plasma glucose and insulin levels, and IR as determined by the homeostasis model assessment (HOMA-IR) (Fig. 1C–E), indicating that resistin induced IR in WT mice.

Figure 1. Effect of resistin on hypertension and insulin resistance in mice.

Figure 1

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(A) Plasma resistin levels were measured in control and resistin-treated (retn) mice after 6 days PBS or resistin treatment; (B) Blood pressure (BP) in wild-type (WT) mice. BP was measured before resistin treatment (day 0, D0) and after 6 days of resistin treatment (day 6, D6); (C) Plasma glucose levels; (D) Plasma insulin levels; (E) HOMA-IR was calculated using the following formula: fasting glucose (mmol/L) × fasting insulin (mU/ml)/22.5. Retn group was injected with 400 ng/day resistin, while the control group was injected with PBS. Injections were performed once per day for 6 consecutive days. Data are presented as mean ± sd (n = 9–10). *P < 0.05, **P < 0.01.

The induction of hypertension and IR by resistin is abrogated in tlr4 −/− mice

TLR4 is a putative resistin receptor; therefore, TLR4 knockout (tlr4−/−) mice were used to determine whether resistin induces hypertension and IR through TLR4. Plasma resistin levels were higher in resistin-treated group than control group, indicating our resistin treatment is successful (Fig. 1A). No significant differences in BP were observed between WT and tlr4−/− mice treated with PBS [Fig. 1B, SBP in WT: 103 ± 6 (Con, D0), 101 ± 13 (Con, D6) mmHg; DBP in WT: 69 ± 11 (Con, D0), 66 ± 8 (Con, D6) mmHg; Fig. 2A, SBP in tlr4−/−: 106 ± 7 (Con, D0), 105 ± 8 (Con, D6) mmHg; DBP in tlr4−/−: 70 ± 13 (Con, D0), 75 ± 9 (Con, D6) mmHg], indicating that TLR4 deficiency had no effect on BP under normal conditions. However, BP values in resistin-treated tlr4−/− mice [Fig. 2A, SBP in resistin-treated tlr4−/−: 103 ± 4 (Retn, D0), 108 ± 9 (Retn, D6) mmHg; DBP in resistin-treated tlr4−/−: 66 ± 8 (Retn, D0), 74 ± 9 (Retn, D6) mmHg] were similar to those observed in PBS-treated tlr4−/− mice [Fig. 2A, SBP in PBS-treated tlr4−/−: 106 ± 7 (Con, D0), 105 ± 8 (Con, D6) mmHg; DBP in PBS-treated tlr4−/−: 70 ± 13 (Con, D0), 75 ± 9 (Con, D6) mmHg], indicating that resistin had no effect on BP in mice lacking TLR4. Moreover, resistin did not affect plasma glucose (Fig. 2B) and insulin (Fig. 2C) levels in tlr4−/− mice, and no differences in HOMA-IR were observed (Fig. 2D). Taken together, these data demonstrate that the action of resistin on hypertension and IR is mediated by TLR4.

Figure 2. Effect of resistin on hypertension and IR in tlr4−/− mice.

Figure 2

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(A) BP in tlr4−/− mice. BP was measured before resistin treatment (day 0, D0) and after 6 days of resistin treatment (day 6, D6); (B) Plasma glucose levels; (C) Plasma insulin levels; (D) HOMA-IR was calculated using the following formula: fasting glucose (mmol/L) × fasting insulin (mU/ml)/22.5. Retn group was injected with 400 ng/day resistin, while the control group was injected with PBS. Injections were performed once per day for 6 consecutive days. Data are presented as mean ± sd (n = 9). *P < 0.05, **P < 0.01.

Resistin activates the renin-angiotensin system by upregulating Agt expression

To investigate the mechanistic basis for resistin-induced hypertension, the mRNA levels of certain BP-regulatory genes were measured. Resistin significantly upregulated Agt mRNA expression in the liver of WT mice, whereas it had no effect in tlr4−/− mice (Fig. 3A). Plasma ANG II level, the active form of Agt, was not significantly different, although a trend toward an increase in resistin-treated mice was observed (Fig. 3B). Similar mRNA levels of angiotensin-converting-enzyme (ACE), endothelial nitric oxide synthase (eNOS), and endothelin receptors A (ETA) and B (ETB) were detected in the lungs of WT and tlr4−/− mice (Fig. 3C–F). In addition, renin (Ren), angiotensin-converting-enzyme 2 (ACE2) and angiotensin II receptor type 1a (Agtr1a) levels were not affected by resistin in WT mice (data not shown). Similar results were obtained in in vitro studies. After 24 h of resistin treatment, Agt mRNA was significantly upregulated in HepG2 cells (Fig. 3H), whereas endothelin-1 (ET-1) and eNOS levels remained constant in EA.hy 926 endothelial cells (Fig. 3I). Subsequently, siRNA was used to inhibit tlr4 expression. After 24 h, Agt expression was detected in HepG2 cells. The data showed siRNA dramatically suppressed tlr4 expression and this effect blocked the stimulation effect of resistin on Agt expression (Fig. 3G–H). These data indicated that resistin specifically stimulates Agt expression in the liver and this effect is TLR4-dependent.

Figure 3. mRNA and plasma levels of BP-regulatory genes.

Figure 3

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mRNA and plasma levels of BP-regulatory genes were measured in WT mice and tlr4−/− mice. (A) Angiotensinogen (Agt) was detected in the liver; (B) plasma ANG II levels; (C) ACE, (D) eNOS, (E) ETA, and (F) ETB were detected in the lung; (G) Inhibition efficiency of tlr4 by siRNA, NC group was transfected with scrambled siRNA; (H) Inhibiting tlr4 blocked the stimulation effect of resistin on Agt expression, NC and resistin groups were transfected with scrambled siRNA; (I) eNOS and ET-1 were measured in EA.hy 926 cells. Data are presented as mean ± sd. (for studies in vivo, n = 3–6; for studies in vitro, 3 independent experiments are performed) **P < 0.01, ***P < 0.001.

As a precursor of Angiotensin I (ANG I), Agt is crucial to the renin-angiotensin system (RAS), which is known as a classical blood pressure regulation system. Therefore, the signal transduction pathway of resistin was further examined by blocking the RAS using the ACE inhibitor perindopril (peri). Resistin had no effect on SBP and DBP in WT mice pre-treated with perindopril (Fig. 4A), and plasma glucose levels were also unchanged (Fig. 4B). Moreover, resistin-induced upregulation of Agt was inhibited by pre-treatment with perindopril (Fig. 4C). These findings indicate that resistin-induced hypertension is dependent on the activation of the RAS.

Figure 4. Perindopril blocks the action of resistin.

Figure 4

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(A) BP in wild-type mice pre-treated with perindopril (Peri). BP was measured before resistin treatment (day 0, D0) and after 6 days of resistin treatment (day 6, D6); (B) Plasma glucose levels in mice exposed to different treatments; (C) Agt and (D) p65 expression in mice exposed to different treatments and in different mouse lines; (E) Binding of p65 to the Agt promoter was determined by chromatin immunoprecipitation. Retn group was injected with 400 ng/day resistin, while the control group was injected with PBS. Perindopril (5 mg/kg/day) was administered orally for 7 days (animals were treated as described in Materials and Methods). Data are presented as mean ± sd (n = 8). *P < 0.05, **P < 0.01.

Resistin induces Agt expression by activating the TLR4/P65 pathway

In our previous study, resistin upregulated p65 mRNA and protein expression13. Consistently, in the present study, hepatic p65 expression was upregulated in WT mice treated with resistin, but not in tlr4−/− or perindopril-treated mice (Fig. 4D). Deb et al. identified a p65 binding site in the mouse Agt promoter, implying that p65 can directly activate Agt transcription14. To determine whether p65 is involved in resistin-induced Agt upregulation, we used ChIP to quantify the occupancy of p65 on the Agt promoter. Our data showed that resistin treatment increased binding of p65 to the Agt promoter in WT mice, but this effect was abolished in tlr4−/− mice (Fig. 4E). These results support that resistin activates the RAS through the TLR4/P65/Agt pathway.

Discussion

Epidemiological studies have demonstrated that patients with diabetes are more likely to develop hypertension, which increases their risk for CVD2. Moreover, as a major pathological feature of type 2 diabetes, IR has often been reported occur in the people with essential hypertension, suggesting these two pathological conditions may develop through a shared pathway3,15. Resistin has been suggested to induce IR and is associated with the development of CVD8. However, the role of resistin in the regulation of BP and the development of diabetes and hypertension remains unclear. In the present study, we investigated the effect of resistin on BP and IR. Our data showed that resistin induces both IR and hypertension in mice and these effects are TLR4-dependent.

Studies in rodents and humans have suggested that resistin promotes IR6,7, although contradictory findings have also been reported16,17. In the present study, plasma glucose and insulin levels, as well as HOMA-IR, were elevated in WT mice after resistin treatment (Fig. 1C–E), confirming that resistin induces IR. To investigate the role of resistin in the development of hypertension, WT and tlr4−/− mice were treated with resistin for 6 days. Both SBP and DBP increased in WT mice, whereas no changes in BP were observed in tlr4−/− mice, indicating that resistin likely regulates BP through TLR4-mediated signaling. In addition, resistin had no effect on plasma glucose and insulin levels or HOMA-IR in tlr4−/− mice (Fig. 2B–D), suggesting that resistin-induced IR is also TLR4-dependent.

Previous studies have implicated resistin in the regulation of certain vasoconstrictors and vasodilators9,18,19,20. Here, we linked resistin with RAS, which is a hormone system involved in BP regulation. As a precursor of ANG II, Agt is released from the liver and converted into ANG I through the action of renin. Then, ANG I is converted to ANG II (a major RAS effector) by ACE21,22. An increase in Agt expression could cause an increase in ANG II and activation of RAS, leading to an elevation in BP. In the present study, Agt expression in the mouse liver was significantly upregulated by resistin treatment (Fig. 3A). Pre-treatment of mice with the ACE inhibitor perindopril abolished resistin-induced hypertension and IR, suggesting that the action of resistin is inhibited when the RAS is blocked. There is considerable evidence demonstrating the activation of the NF-κB pathway by resistin23,24,25 and resistin was previously found to enhance p65 mRNA and protein expression in the liver13. As a major transcription factor in the NF-κB pathway, p65 (Rel A) can directly bind to the promoter of Agt and thereby upregulate Agt expression14. Therefore, we presumed that resistin-induced upregulation of Agt expression could be mediated by p65. Quantification of mRNA levels indicated that resistin upregulated the expression of p65 in WT mice, but not in tlr4−/− or perindopril-treated mice (Fig. 4D), corresponding to the upregulation of Agt expression. ChIP data showed that resistin promoted the binding of p65 to the Agt promoter in WT but not tlr4−/− mice (Fig. 4E), indicating that resistin upregulates Agt expression via the TLR4/p65 pathway.

Unlike mice, in human, resistin is produced mainly in macrophages rather than in adipocytes26, therefore, whether its effect on rodents is translatable to human is not fully confirmed27,28. However, an elevated resistin level has been observed in the patient with IR or type 2 diabetes in many clinical studies and in the patients with essential hypertension according to some studies29,30. This also fits to the finding in our mouse model on the importance of resistin linking IR and hypertension. However, dual to the complexity in human epidemiology, it’s difficult to address whether resistin is an active player in the development of those pathological conditions especially hypertension in diabetes patients. Therefore, some literatures suggest effect of resistin on diabetic hypertension may be through elevated ET-1 or reduced eNOS31,32 which we didn’t observed in our mouse model (Fig. 3D) as well as in vitro study (Fig 3F). Here, we have found the role of resistin in the activation of RAS in our mouse model which offered another possible explanation for the association of resistin with diabetes and hypertension.

Taken together, our data indicate that resistin induces IR and hypertension in mice via a mechanism dependent on TLR4 and RAS, suggesting that resistin is a crucial factor linking diabetes and hypertension. The results of the present study support the existence of a common pathway underlying the development of diabetes and hypertension, and provide a novel animal model to further investigate this issue.

Methods

Animals

Male C57BL/10 (wild-type, WT) and C57BL/10ScN (TLR4 gene deleted type, tlr4−/−) mice (7–8 weeks old) received daily intravenous injections of PBS or 400 ng/day of recombinant mouse resistin (R&D Systems, Minneapolis, MN, USA) for six consecutive days. For the renin-angiotensin system (RAS) blocking experiment, mice were pre-treated one day prior to the start of the injection regimen with the angiotensin-converting enzyme (ACE) inhibitor perindopril (5 mg/kg; mBbio, Nanjing, China) by oral administration, and perindopril was administered 30 min before each injection. BP was measured with a tail-cuff system (BP-98A; Gene & I Co. Ltd, Beijing, China). Retro-orbital blood was collected after 12 h of food withdrawal and mice were directly sacrificed afterwards. Plasma glucose levels were determined by the glucose oxidase method using a glucose determination kit (Applygen Technologies Inc, Beijing, China). Insulin and resistin levels were measured with an insulin ELISA kit (Xinqidi Biological Technology Co. Ltd, Wuhan, China).All procedures were performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of Hubei Province. All animal experiments were performed under approval of the Institutional Animal Care and Use Committee (IACUC) (protocol: 00116272) of Wuhan University. The protocol was approved by the Committee on the Ethics of Animal Experiments of Wuhan University (Permit Number: 2013030).

Cell Culture and Treatment

Agt gene expression was assessed in HepG2 cells, and nitric oxide synthase (NOS) and endothelin-1 (ET-1) were assessed in EA.hy 926 endothelial cells. Cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum and cultured under 5% CO2 at 37 °C. The control group was cultured without recombinant resistin, whereas the treatment group was cultured with recombinant resistin (50 ng/mL). Cells were collected 24 h after treatment for RNA isolation.

RNA extractNion and cDNA synthesis

Total RNA was isolated with TRIzol (Takara, Dalian, China) according to the manufacturer’s protocol. All RNA samples were treated with DNase I to remove genomic DNA prior to cDNA synthesis. RNA concentration was determined using a SMA4000 UV-Vis Spectrophotometer (Merinton, Beijing, China). Total RNA was reverse-transcribed using a cDNA Synthesis kit (Takara, Dalian, China).

Real-time PCR

For determination of gene expression in mice, the β-actin gene was used as the internal control; for gene expression in HepG2 cells and EA.hy 926 endothelial cells, the GAPDH gene was used as the internal control. Primer sequences and accession numbers for each gene are listed in Supplemental Table S1.

Chromatin immunoprecipitation (ChIP)

ChIP was performed using the SimpleChIP Plus Enzymatic Chromatin IP Kit (Magnetic Beads) (Cell Signaling Technology, Inc., Beverly, MA, USA) following the manufacturer’s instructions. For each immunoprecipitation reaction, 5 μg of mouse P65 antibody (Santa Cruz, CA, USA) was used for every 5 μg of pre-cleared chromatin. Recovered DNA was quantified by real-time PCR. The primer sequences for amplifying P65 binding sites on the mouse Agt promoter have been previously described14.

Statistical analysis

Results are presented as the mean ± sd. Statistical analyses were performed using an unpaired two-tailed t-test, or ANOVA for more than two groups. The correlation was estimated by partial correlation analysis after adjustment for gender, age, and body weight. P < 0.05 was considered statistically significant.

Additional Information

How to cite this article: Jiang, Y. et al. Resistin Induces Hypertension and Insulin Resistance in Mice via a TLR4-Dependent Pathway. Sci. Rep. 6, 22193; doi: 10.1038/srep22193 (2016).

Supplementary Material

Supplementary Table
srep22193-s1.doc (41KB, doc)

Acknowledgments

This work was supported by the grants from National key Basic Research Program of China (2012CB124702), 948 Program (2015-Z33), National Natural Science Foundation of China (31172093, 31301947), the National Science Foundation for Fostering Talents in Basic Research (J1103510), Specialized Research Fund for the Doctoral Program of Higher Education (20110146130002), the Fundamental Research Funds for the Central Universities (2013PY005), and Guangxi Natural Science Foundation (2014GXNSFDA118014).

Footnotes

Author Contributions Y.J., L.L., Y.H., Q.L., C.A., X.Y., L.S., A.C. and C.N. performed experiments. L.Z. and Z.Y. conceived and supervised the study. Y.J., L.Z. and Z.Y. wrote the paper. All authors reviewed the manuscript.

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Associated Data

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Supplementary Materials

Supplementary Table
srep22193-s1.doc (41KB, doc)

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