Skip to main content
PMC home page
Journal of Clinical Pathology logoLink to Journal of Clinical Pathology
. 1970 Oct;23(7):558–562. doi: 10.1136/jcp.23.7.558

An assessment of serum 57Co cyanocobalamin as an index of vitamin B12 absorption1

D Donaldson 1,2, B J N Blight 1,2, P T Lascelles 1,2
PMCID: PMC476835  PMID: 5483382

Abstract

A technique has been developed for accurately assaying serum and urine 57Co cyanocobalamin during the conventional Schilling test. Serum and urine radioactivity in a large number of patients has been correlated and the precise normal limits have been determined by statistical analysis. The implications of this for investigating conditions other than pernicious anaemia and the malabsorption syndromes are stressed.

Full text

PDF
558

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADAMS J. F., CARTWRIGHT E. J. The reliability and reproducibility of the Schilling test in primary malabsorptive disease and after partial gastrectomy. Gut. 1963 Mar;4:32–36. doi: 10.1136/gut.4.1.32. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ADAMS J. F., SEATON D. A. Reproducibility and reliability of the Schilling test. J Lab Clin Med. 1961 Jul;58:67–75. [PubMed] [Google Scholar]
  3. BOOTH C. C., MOLLIN D. L. Plasma, tissue and urinary radioactivity after oral administration of 56Co-labelled vitamin B12. Br J Haematol. 1956 Jul;2(3):223–236. doi: 10.1111/j.1365-2141.1956.tb06695.x. [DOI] [PubMed] [Google Scholar]
  4. Coupland W. W. Plasma radioactivity after radioactive vitamin B12 given orally. Med J Aust. 1966 Jun 11;1(24):1020–1023. doi: 10.5694/j.1326-5377.1966.tb73202.x. [DOI] [PubMed] [Google Scholar]
  5. DOSCHERHOLMEN A., HAGEN P. S. Radioactive vitamin B12 absorption studies: results of direct measurement of radioactivity in the blood. Blood. 1957 Apr;12(4):336–346. [PubMed] [Google Scholar]
  6. Donaldson D., Lascelles P. T. Vitamin B12 absorption in some neurological and neuroendocrine disorders. J Clin Pathol. 1970 Oct;23(7):563–568. doi: 10.1136/jcp.23.7.563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Finney R. D., Payne R. W. Plasma radioactivity following oral administration of 57Co-labelled vitamin B 12. Scott Med J. 1968 Dec;13(12):425–429. doi: 10.1177/003693306801301204. [DOI] [PubMed] [Google Scholar]
  8. Forshaw J., Harwood L. Measurement of intestinal absorption of 57-Co vitamin B-12 by serum counting. J Clin Pathol. 1966 Nov;19(6):606–609. doi: 10.1136/jcp.19.6.606. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GLASS G. B. J., BOYD L. J., GELLIN G. A., STEPHANSON L. Uptake of radioactive vitamin B12 by the liver in humans: test for measurement of intestinal absorption of vitamin B12 and intrinsic factor activity. Arch Biochem Biophys. 1954 Jul;51(1):251–257. doi: 10.1016/0003-9861(54)90472-5. [DOI] [PubMed] [Google Scholar]
  10. MCCURDY P. R. THE DETECTION OF INTESTINAL ABSORPTION OF CO57 TAGGED VITAMIN B12 BY SERUM COUNTING. Ann Intern Med. 1965 Jan;62:97–102. doi: 10.7326/0003-4819-62-1-97. [DOI] [PubMed] [Google Scholar]
  11. McIntyre P. A., Wagner H. N., Jr Comparison of the urinary excretion and 8 hour plasma tests for vitamin B12 absorption. J Lab Clin Med. 1966 Dec;68(6):966–971. [PubMed] [Google Scholar]
  12. Meyer B. J., Jansen C. R., van der Merwe C. J., Oosthuizen S. F. Application of whole-body gamma spectrometry to vitamin B12 absorption. S Afr Med J. 1968 Jan 20;42(3):50–52. [PubMed] [Google Scholar]
  13. NELP W. B., McAFEE J. G., WAGNER H. N., Jr Single measurement of plasma radioactive vitamin B12 as a test for pernicious anemia. J Lab Clin Med. 1963 Jan;61:158–165. [PubMed] [Google Scholar]
  14. REIZENSTEIN P. G., CRONKITE E. P., COHN S. H. Measurement of absorption of vitamin B12 by whole-body gamma spectrometry. Blood. 1961 Jul;18:95–101. [PubMed] [Google Scholar]
  15. SCHILLING R. F. Intrinsic factor studies II. The effect of gastric juice on the urinary excretion of radioactivity after the oral administration of radioactive vitamin B12. J Lab Clin Med. 1953 Dec;42(6):860–866. [PubMed] [Google Scholar]
  16. Saif M. The validity of the Schilling test in investigating the intestinal absorption of vitamin B12 in Eqyptians. I. Normal individuals. J Egypt Med Assoc. 1967;50(11):714–718. [PubMed] [Google Scholar]
  17. Townsend J. D., Smith T., Langman M. J. Reproducibility of the hepatic uptake test of vitamin B12 absorption. Gut. 1968 Apr;9(2):199–202. doi: 10.1136/gut.9.2.199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. VELDEK T. E., ABELS J., ANDERS G. J., ARENDS A., HOEDEMAEKER P. J., NIEWEG H. O. A FAMILY STUDY OF PERNICIOUS ANEMIA BY AN IMMUNOLOGIC METHOD. J Lab Clin Med. 1964 Aug;64:177–187. [PubMed] [Google Scholar]
  19. Wangel A. G., Callender S. T., Spray G. H., Wright R. A family study of pernicious anaemia. II. Intrinsic factor secretion, vitamin B12 absorption and genetic aspects of gastric autoimmunity. Br J Haematol. 1968 Feb;14(2):183–204. doi: 10.1111/j.1365-2141.1968.tb01486.x. [DOI] [PubMed] [Google Scholar]
  20. Whittingham S., Ungar B., Mackay I. R., Mathews J. D. The genetic factor in pernicious anaemia. A family study in patients with gastritis. Lancet. 1969 May 10;1(7602):951–954. doi: 10.1016/s0140-6736(69)91856-x. [DOI] [PubMed] [Google Scholar]
  21. Woodliff H. J., Armstrong B. K. Vitamin B12 absorption studies: comparison of 57Co vitamin B12 plasma levels, urinary excretion and faecal excretion. Med J Aust. 1966 Jun 11;1(24):1023–1025. doi: 10.5694/j.1326-5377.1966.tb73203.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Pathology are provided here courtesy of BMJ Publishing Group

RESOURCES