Successful Direct Adsorption of Lipoproteins (DALI) Apheresis During Pregnancy in an Omani Woman with Homozygous Familial Hypercholesterolemia

Abstract

We report our experience with Direct Adsorption of Lipoproteins (DALI) apheresis in an Omani pregnant woman affected by homozygous familial hypercholesterolemia. To the best of our knowledge this is the first successful pregnancy treated with DALI apheresis.

The patient had a history of coronary artery disease, supra-aortic valvular stenosis and severe carotid artery disease with right carotid artery stenting. She was on a regular biweekly DALI apheresis since 2008. In May 2013, she became pregnant and rosuvastatin and ezetimibe were stopped while she continued on DALI apheresis biweekly. This treatment during pregnancy was successful with no major complications. The average low-density lipoprotein cholesterol reduction during therapy was 50%. She spontaneously delivered a healthy male infant (2,400 g) at 37 weeks. We showed that DALI apheresis therapy was safe during pregnancy with a good outcome for both mother and neonate.

INTRODUCTION

Homozygous Familial Hypercholesterolemia (HoFH) is an inherited disease caused by genetic defects in low-density lipoprotein receptors (LDLRs), apolipoprotein B (Apo B) and proprotein convertase subtilisin/kexin type 9 (PCSK9) [1, 2] HoFH is characterized by very high levels of low-density lipoprotein cholesterol (LDL-C) and if not recognized and treated early it will lead to severe cardiovascular disease (CVD) early in life [3-5] HoFH is treated by combination of statin with or without ezetimibe and regular LDL apheresis [6-5].

High concentrations of LDL-C levels during pregnancy may have serious consequences for both the fetus and mother. It may increase the risk of acute myocardial ischemia for women who have underlying coronary artery disease (CAD)[6-19] and it may cause fetal intrauterine growth restriction [20-22]. The use of statins is contraindicated during pregnancy and LDL apheresis should be considered in the management of pregnant women with HoFH.

There are several reported cases of successful pregnancy with FH treated with plasma exchange [23, 24], heparin extracorporeal LDL-C precipitation (HELP) [25, 26], or dextran sulphate-cellulose adsorption (DSA) [27, 28], cascade filtration system [29] and immunoadsorption (IA) [30] apheresis. For direct adsorption of lipoproteins (DALI) apheresis, there was one reported case of apheresis therapy during pregnancy but the treatment was not successful and was stopped at 28 weeks because of severe tetany related to treatement [31]. To our knowledge, we report the first successful pregnancy involving a HoFH patient treated with DALI apheresis Fig. (1) and Fig. (2).

Fig. (1).

Lipid levels pre and post LDL-apheresis before, during and after pregnancy.

Fig. (2).

Apo B levels pre and post LDL-apheresis before, during and after pregnancy.

CASE REPORT

The patient was a 38-year old woman diagnosed with HoFH with LDL-receptor mutation at exon 4 G397A and PCSK9 mutation at exon 9 A1420G. She was on rosuvastatin 40 mg and ezetimibe 10 mg. In addition, she was on biweekly DALI LDL-apheresis using a left arm basilic arterio-venous (A-V) shunt. She had carotid artery disease with a right carotid endarterectomy in 2004 and right carotid stent. She had moderate supravalvular aortic stenosis on echocardiogram but with normal left ventricular function and evidence of CAD on angiography in 2006 with minimal left anterior descending disease. The patient remained asymptomatic through the years and responded very well to pharmacological treatment and LDL-apheresis.

Prior to this pregnancy, she had 2 live children in good health but had lost 3 pregnancies. When she became pregnant this time, rosuvastatin and ezetimibe were discontinued while she was continued on low fat diet. Her pregnancy was managed by a multidisciplinary team consisting of obstetric, cardiology and lipid specialists. Due to her high risk background and LDL-C of 14.4 mmol/L, she was continued on LDL-apheresis. All the biweekly DALI were performed with the DALI 750 adsorbers (Fresenius HemoCare Adsorber Technology GmbH, St. Wendel, Germany). In each session, an average of 7,100 ml of whole blood was processed in about 2 h, at an average rate of 60 ml/h. Anticoagulation in the extracorporeal system was carried out by acid citrate dextrose (ACD-A) infusion and was first mixed with the patient’s blood at a ratio of 1:20 and reduced to 1:40 after 1,500 ml of blood was treated. Average calcium levels were 2.43 mmol/L and the patient was receiving oral calcium supplements before each apheresis therapy. Fetal monitoring was performed before and after DALI apheresis and no fetal heart rate abnormalities were detected. The first DALI-apheresis resulted in a 54% reduction of LDL-C levels to 6.6 mmol/L (Table 1).

Table 1.

Baseline lipid profile for the family.

	TC mmol/L	LDL-C mmol/L	HDL-C mmol/L	TG mmol/L	Apo B g/L	Apo A1 g/L
Husband	6.0	3.8	1.08	2.3	1.19	1.38
Wife (patient)	16.9	14.4	1.67	2.0	3.97	1.79
Daughter 1	7.4	5.6	0.98	1.8	1.52	1.19
Daughter 2	7.7	5.6	1.24	1.7	1.52	1.41
Daughter 3	6.7	5.0	1.38	0.9	1.26	1.33
Son 1	8.4	6.1	1.10	2.8	1.69	1.18
Son 2 (New born)	4.1	1.9	0.73	3.3	0.87	1.19

The patient continued to receive DALI-apheresis until 37 weeks of gestation with average blood pressure (BP) 132/54 mmHg, blood glucose 5.1 mmol/L, glycosylated hemoglobin (HbA_1C) 6.2%, weight 82 Kg and body mass index (BMI) 36. She had uncomplicated spontaneous vaginal delivery of a healthy boy weighing 2,400 g. From Week 13 to 37 of her pregnancy, the patient received 12 DALI-apheresis to control cholesterol levels.

DISCUSSION

Pregnancy in women with HoFH carries risk to the mother and fetus. High maternal cholesterol may cause growth restriction, preeclampsia and miscarriage in animal and human studies [32, 33]. Pregnancy in women with HoFH is contraindicated and there are no clear guidelines for their management. Nevertheless, there are several reported cases in the literature of women treated with apheresis using plasma exchange, HELP, DSA, cascade filtration system and IA apheresis [23-30].

This is the first case of successful pregnancy outcome using the DALI apheresis system. A previous report with DALI apheresis was not successful and the apheresis was stopped at 28 weeks of gestation due to severe tetany but the pregnancy was carried out to term [31]. In our case and prior to the current pregnancy and the commencement of DALI apheresis, the woman had 2 healthy babies and 3 miscarriages. She was diagnosed with severe carotid artery disease, moderate supravalvular aortic stenosis and mild CAD. Although, she was counseled against undertaking pregnancy the decision after she became pregnant was to provide appropriate management of her cholesterol levels and the risk prevent further worsening of her cardiovascular disease.

The most suitable anticoagulants for apheresis during pregnancy are unfractionated low molecular weight heparin and citrate. Low molecular weight heparin has fewer side effects, is well tolerated and does not pass the blood-brain barrier [34]. In addition, apheresis or plasma exchange with citrate was also used in pregnant women [35, 36]. In our case the use of ACD-A infusion as anticoagulant was proved to be safe with very minimal side effects of paresthesia around the mouth and the jaw during the sessions and this did not result in discontinuation of treatment.

In our lipid and LDL apheresis unit at Sultan Qaboos University hospital in Oman we have identified 5 HoFH with molecular genetic confirmation and we have reported previously 2 of these cases including the current reported case [37-39]. We had lost one of the HoFH who was a female child with very severe HoFH and severe atherosclerosis. Currently four HoFH, one heterozygous FH (HeFH) with severe CAD and one HeFH with statin intolerance and CAD are receiving regular DALI apheresis in our unit.

In conclusion, this is the first case report of a successful pregnancy outcome in a patient with HoFH treated with DALI apheresis. In general, although pregnancy and delivery in women with HoFH and cardiovascular disease are hazardous and should be monitored very closely, long-term LDL apheresis seems feasible to control superimposed hyperlipidemia, cardiovascular disease, placental insuffici-ency and subsequent intrauterine growth restriction.

CONFLICT OF INTEREST

The authors confirm that this article content has no conflict of interest.